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In the paper we evaluate the influence of crown thinning with positive selection, different intensity (moderate PRP III and heavy PRP IV) and 5-year frequency on development of growth, production, quality and leaf area index of black walnut (Juglans nigra L.) monocultures growing on the series of three permanent research plots (PRP) Sikenica (Levice Forest Enterprise, Levice Forest District) as observed in 1978-2003. The trends of development of mean stem, basal area, standing volume and aboveground dendromass (in dry matter) were mainly influenced by heavy crown thinning. The index of growth was as follows: basal area 169.01%, standing volume 262.12%, aboveground dendromass (in dry matter) 324.48%. At the age of 64 years the black walnut monocultures tended by heavy crown thinning had the following parameters: basal area 31.03 m²/ha, standing volume 463.88 m³/ha and aboveground dendromass 194.98 t/ha. Mean periodic increment reached the values: basal area 0.51 m²/ha/year, standing volume 11.48 m³/ha/year and dendromass 5.39 t/ha/year. The index of increment percent growth was: basal area + 31.75%, growing stock + 30.85% and dendromass + 0.79%, compared to the control. The total production was also significantly influenced by heavy thinning. At the stand age of 64 years the tended stands had the total basal area of 4.92 m²/ha, total volume production of 572.77 m³/ha and total weight production of 246.04 t/ha. The total mean increment of basal area is 0.67 m²/ha/year, of volume 8.95 m³/ha/year and of weight 3.84 t/ha/year, which is by 24.07%, 23.96% and 16.01% more than on the control plot. The leaf area index at the age of 64 years ranges from 6.54 ha/ha (PRP III) to 7.82 ha/ha (PRP V). Dendrochronological analyses revealed minimum widths of annual rings in the years 1952, 1961, 1968, 1971, 1975, 1981, 1983, 1985, 1993, 2000, maximum ones in 1951, 1957, 1959, 1967, 1974, 1979, 1982, 1984, 1989, 1999.

Norway spruce yellowing and defoliation caused by Mg deficiency was frequently recorded on many sites of the Šumava Mts. (Bohemian Forest Mts.) since 1980's. A forest experiment aimed at investigation response of spruce to Mg application (commercial fertilizer SILVAMIX^® Mg NPK) was set up in 1998. Fertilizer was applied manually in a dose of 96.5 kg Mg/ha in spruce (S) and beech-spruce (BS) vegetation altitudinal zones in spring 2000. Immediate decrease of yellowing was revealed in fertilized plots in both vegetation zones and yellowing almost completely disappeared at the end of the investigation in 2005. Further, fertilization resulted in stabile foliation while marked defoliation was reported from control plots in both vegetation zones. Mg deficiency can be effectively eliminated by appropriate fertilizer application. Balanced nutrition contributes to long-term vigour and stability of forest stands.

Previous experiences with breeding of lime tree (Tilia cordata Miller) in European countries are shown in this paper. The first results were obtained from the evaluation of juvenile planting of lime tree (Tilia cordata Miller) in the Czech Republic, founded in the School Forest Enterprise Kostelec nad Černými lesy. Lime tree clones (initially cultivated in vitro) were evaluated for growth parameters and also according to the growth form of the different clones. Individual clones were also checked for mortality.

The second part of the paper summarizes results of an institutional research aimed at the determination of physical and mechanical properties of different sets of plywood sheets pressed under different conditions. The first part dealt with the determination of compressibility or values of decreasing the thickness of pressed plywood sheets. In this second part, results are summarized of the analysis of physical and mechanical properties of the set of whole-beech plywood sheets of the nominal thickness of veneers amounting to 1.5 mm. The plywood sheets were manufactured as seven-ply and urea-formaldehyde resin DUKOL S was used for their production. The sheets were pressed using a pressure of 1.5 and 1.7 MPa. Following parameters were analyzed: moisture, density, bending strength, MOE in bending and shear strength.

This study was designed to evaluate basic tree characteristics of Quercus dalechampii Ten. and Quercus polycarpa Schur and to find out differences between them. Total height, height of crown base and diameter at breast height were measured before tree felling. Cut stems were visually checked for heart rot on their basal parts. Diameters were measured on each stem in 1 meter long sections from the base to the point of life crown setting. Average values for both species were compared by t-test. The total height was proved to be statistically different; the height of crown base and diameter at breast height were quite similar for both species. Q. dalechampii Ten. was proved to be of larger stem diameter from the tree foot to the 3^rd height meter; the diameter of Q. polycarpa Schur was superior from that height upwards. However, the most significant interspecies difference was found in a number of stems affected by wood-rot fungi as Q. polycarpa Schur turned out to be less resistant than Q. dalechampii Ten.

The paper deals with problems of determination of imagoes (mainly females) in bark beetles of the genus Pityokteines Fuchs. P. curvidens (Germ.) is on average only slightly longer than P. spinidens (Reitt.) and distinctly longer than P. vorontzowi (Jak.). Males are (without lower hooked teeth) equally long as females. The shield of P. curvidens is on average 8% (P. spinidens 12% and P. vorontzowi 13%) longer than wider. Males of P. curvidens have wing cases at shoulders equally wide as their shield, females on average 3.3% wider than their shield. Males of P. spinidens have wing cases on average 1.0% narrower (females 2.1% wider) than their shield. Males of P. vorontzowi have wing cases on average 1.3% narrower than their shield, females have wing cases equally wide as their shield. The longest tail-like hair of the shield front edge (about 0.38 mm) occurs in females of P. spinidens, much more shorter (about 0.26 mm) in P. curvidens and shortest (about 0.23 mm) in P. vorontzowi. In females of P. curvidens, the hair of forehead as against the hair of shield is shorter on average by 8.3, in P. spinidens by 5.6 and in P. vorontzowi by 9.5%. Based on the length of the shield hair it is possible to distinguish reliably females of P. spinidens and according to short (0.12 mm) bristles on the rear of wing cases females of P. vorontzowi. A longitudinal central keel occurs on the forehead of 80% males of all three species and 72% females of P. vorontzowi. A figure defined by the teeth of the 2^nd and the 5^th pair in females of P. curvidens on the rear of wing cases is an isosceles trapezoid that approaches (or is almost equal) to a rectangle. In P. spinidens, it is quite a well-marked (in P. vorontzowi well-marked) trapezoid. It has been found that the shape and size of these figures are the main characteristics for species determination of females of the genus Pityokteines.

This study deals with the assessment of increment losses in Scots pine trees caused by the maturation feeding of pine shoot beetles Tomicus piniperda (L.) and T. minor (Hart.) (Col., Scolytidae) in even-aged stands growing during their entire life span within range of the influence of a sawmill and its timber yard. In spring 2004, on three sample plots, 0.2 ha in size, situated 60, 200, and 500 m from the yard, height and dbh of all trees were measured and increment cores were taken from randomly selected sample trees. An agreement between the spatial distribution of losses in tree increments and the distribution of damage to crowns of investigated stands indicated that the losses resulted from the maturation feeding of pine shoot beetles migrating from the sawmill timber yard. Divergence of growth in the about 25-years-old stand indicated the beginning of intensive feeding of beetles in pine shoots with a high level of probability. In comparison with the control stand the basal area losses in stands growing 60 and 200 m from the beetle source amounted to 57% and 46%, respectively. The difference in the height of trees was as large as almost 100%.

Forestreclamation of spoil banks in mining areas in the North-West of the country started in the second half of the last century. Nowadays forest stands growing on anthropogenic substrates which are still unlike to forest soils, are getting in the phase of possible natural and/or artificial regeneration. The basic characteristics of substrate in the area and ecological attitude of used tree species are driving variables for successful regenerations. The study summarizes the vitality and growth of selected species on anthropogenic substrates in the area with the recommendations of key tree species used for forest reclamation. The results show the best adaptation to anthropogenic substrates for black alder and maple.

The Pinus mugo complex (P. mugo Turra sensu lato, and/or species aggregate or group - P. mugo agg., including its internal as well as external aggregate hybrids) is an important fragment of European dendroflora. For its high variability this complicated group has not been solved in taxonomical terms satisfactorily yet. Most problems are mainly caused by a large amount of taxa of uncertain value described in the past, which often more or less overlap or are based on insufficiently researched and unsuitably interpreted characters. Such facts coincidently cause further problems in applied disciplines. Specialized European literature already contains a large amount of information referring to individual taxa of this complex. Much information is listed under various, often commonly not known or not used scientific names. In order to make the information available and useful, it was necessary to collect the most frequent names used in the past and to assort them in a tabular manner into a convenient synoptic schedule respecting the present concepts of the complex and assign them with the present most commonly used names.

The paper deals with the study of bionomics and population dynamics of Ips typographus (L.) in the area of the Praděd National Nature Reserve (NNR). Data were collected on the basis of detected feeding marks, beetles caught in traps, and also according to the frequency of occurrence or increase in the number of standing dead trees killed by the bark beetle attack in localities where sanitation felling was not performed. Finally, evaluation of the present condition of forest stand and population dynamics of Ips typographus was carried out. The results showed that the stands currently occur in the stage of disintegration when the role of the bark beetle is irreplaceable. Based on the research findings, the management of the bark beetle was proposed that would enable to achieve so-called regulated disintegration of natural spruce forests in the area of the Praděd NNR.

Dokumenty týkajúce sa regulácie hospodárenia v lesoch sa prakticky až dodnes považovali za materiály, týkajúce sa skoro výlučne len jednotlivých odborných rezortov. Na základe Vyhlášky Ministerstva financií SR č. 465/1991 ich možno efektívne využívať aj v rámci rezortov financií a spravodlivosti. Ďalšie významné medzirezortné využitie týchto dokumentov vyplýva zo Zákona č. 127/1994 o posudzovaní environmentálneho dopadu. Cieľom práce je demonštrovať proces posudzovania na konkrétnom príklade - ploche patriacej do lesného pozemkového fondu. Okrem máp, výsledkov pozorovania, slovných popisov a súborov numerických údajov, týkajúcich sa jednotlivých porastov, obsahujú tieto dokumenty aj kódované údaje, ktoré pre väčšinu užívateľov nie sú zrozumiteľné bez využitia dekódovacích kľúčov. A práve tieto posledné údaje majú pri vyhodnocovaní environmentálneho dopadu najvyššiu výpovednú hodnotu. Po nadobudnutí platnosti pripravovaného zákona (vyhlášky) o strate vlastníctva, vyplývajúcej z prohibitívnych a ďalších podmienok v lesnom hospodárstve v súvislosti s presadzovaním verejno-prospešného záujmu (Kundrík et al. 1999), významne vzrastie dôležitosť využitia finálnych dokumentov týkajúcich sa regulácie hospodárenia v lesoch SR.

Damage to forest stands and their decline in the Czech Republic evoked a necessity to elucidate the function of soil in these processes. Main factors affecting acidification of forest soils are assessed in the paper. On the one hand, it refers to natural factors such as properties of soil-forming substrates and species composition of forest stands, on the other hand, to the effects of anthropogenic character such as influences of man-made spruce monocultures and effects of acid depositions. Special attention is paid to the development of forest soils in the Krušné hory Mts., where the effects of acid depositions are highest.

Harvester technologies represent the second most common logging system in the Czech Republic. The high productivity of this technology is very necessary to cover its acquisition and operational costs. A human factor - a machine operator is the most important factor that surely influences the machine productivity. That is why the aim of this study is as follows: to analyze the mutual dependence of machine operator's education and working experience and harvester and forwarder productivity. The analysis proves that the operator's education and even more his working experience are very important. The time of harvesting work stages was measured when the harvester was operated by an operator with two-year working experience and by an operator with no experience. The average time difference between the harvester operators was 64.9 seconds in one work stage. The statistical significance of different operation times was confirmed during technically demanding segments in the working stage (the boom out the felling head and tree processing). Operators with the same working experience in forestry mechanization - about 13 years but with different education carried out the haulage work stage. In this case, the statistical significance between calculated differences was not proved. This fact proves that the operator's working experience is more important than his education.

The population dynamics of the horse-chestnut leafminer Cameraria ohridella Deschka et Dimić. was investigated in 1997-2000. The mortality of wintering pupae and the rate of fungal infections were also examined. The mortality of the horse-chestnut leafminer was nearly 40% at the end of spring. The spectrum of fungi on examined dead pupae was as follows: Verticillium lecanii (Zimmerman) Viégas, Cladosporium herbarum (Persoon: Fries) Link, Cladosporium cladosporioides (Fresen.) de Vries, Alternaria alternata (Fr.) Keissler, Acremonium strictum W. Gams, Rhizopus stolonifer (Ehrenberg: Fries) Lind., Arthrinium phaeospermum (Corda) Ellis and Acremonium sp. 1. Verticillium lecanii was the only identified fungus with an entomophagous status. The fungus is, however, a well documented entomopathogen of Homoptera, particularly of aphids, scale insects and whiteflies in tropical and subtropical regions.

The article investigates the socio-economic importance of collection and use of non-wood forest products (NWFP) for inhabitants in the Czech Republic (CR) in 2005 and brings a comparison with former investigations and research projects. The research project consisted of these parts in 2005: 1. Analysis of hitherto performed research projects and their results in 1999-2003, 2. Investigation of quantities and values of NWFP collected in 2004 and 2005, 3. Specific investigation of socio-economic importance of NWFP collection and use. The results of respective investigations prove NWFP collection being quite important for the inhabitants of the CR. The importance consists in the material value of picked commodities on the average annual level higher than 3,000 mil. CZK. Nevertheless, collecting NWFP is considered as a very important recreational activity. Only a small part of the population collecting NWFP (about 12%) collects NWFP to save money.

At the end of nineties, within the mountain forests of the Krušné hory Mts., in connection with an increased acid deposition, the symptoms (yellowing) started to be visible, characterizing magnesium deficiency in the assimilation organs, when Mg contents were laying under 300 mg/kg. In mineral soil the Mg content is mostly less than 10 mg/kg. Contents of other basic elements (Ca, K) were also very low. During four years yellowing symptoms of the spruce stands have developed in an extent area of more that 10,000 ha. Since 1999, preventive measures have been adopted to improve the nutrition status of the forest stands. Liquid magnesium fertilizers as MgNsol, Lamag Mg, MgSsol were applied during the period of 1999-2002. Since 2001, in semi-operational experiment, powder Mg fertilizer - Silvamix F4 - of higher Mg content (63.3% MgO) was applied. Application of liquid fertilizer MgNsol has helped to compensate the nitrogen deficiency in the stands of insufficient nutrition in this element. Comparing of different types of liquid fertilizers has confirmed, that the highest increase of magnesium in assimilation organs was find in combined application of MgNsol and MgSsol fertilizers. Application of 300 kg/ha Silvamix F4 has increased the magnesium content in the first needle year class in 73% in average, potassium in 33%, calcium in 16%, and phosphorus in 85%. Besides increased nutrient content in needles also improvement of the health and phosphorus in 85%. Besides increased nutrient content in needles also improvement of the health state, i.e. revitalization of the stands affected can be observed.

The population dynamics of wood mouse (Apodemus sylvaticus) was studied in three forest complexes differing in food supply in the intensively managed landscape of southern Moravia. They included an old semi-natural floodplain forest dominated by oak (HL), production broadleaved forest with the predominance of oak and robinia (HA) and a pheasantry with various forest stands consisting of diverse species and age categories of trees with a quantity of additional food for additional feeding of pheasants and roe deer (RB). The population fluctuation within six-year monitoring was affected by seed years (acorn crops in 2003 and 2006), which resulted in an increase in the species population density in the next year. Statistically significant effects of the crop of acorns on the body weight of monitored species were also detected (P < 0.01, F = 1.44). Relative abundance differed significantly between the floodplain forest (HL) and RB and HA (P < 0.01). The two last-mentioned sites did not differ and only a trend of greater preference to the most variable biotope in RB was noted. It evidences the unsuitability of floodplain forest for the species. In spite of the food specialization in small seeds significant effects of the excess crop of acorns can result in a rapid increase in the Apodemus sylvaticus population which can then cause damage to the natural regeneration of oak or artificial regeneration by seeding although to a substantially smaller extent than the more harmful A. flavicollis.

The subject of our research was to verify effects of the mechanical preparation of soil in the natural regeneration of pedunculate oak in a floodplain forest. On the site of a hard-wooded floodplain forest at the confluence of the Morava and Dyje rivers (Czech Republic), several sample plots were established differing in the preparation of soil during regeneration, in weeding the plots and in the period of the implementation of soil surface scarification before and after the fall (or distribution) of acorns. On the basis of results obtained, mechanical preparation of soil on non-weed-infested plots in the heavy seed year is not necessary, however, it supports seedling emergence from acorns and eliminates weed tree emergence and weed. However, it is better to realize it only after the fall of acorns and to incorporate them into soil and thus their emergence will be increased. Soil scarification can be carried out also in the course of harvesting the parent stand through the extraction of wood and slash from the regenerating area. In weed-infested stands, however, the thorough preparation of soil before the fall of acorns appears to be a necessity.

Results obtained during 14 years of the experiment are presented. Extreme site conditions are determined by: 8^th vegetation altitudinal zone, podzolic soil and hard climate - average annual temperature 4°C, average annual precipitation around 1,200 mm. Effects of surface experimental liming were studied on immission clear-cuts in long time period, study area is located on the Velká Deštná locality on the main Orlické hory Mts. range. Research plots were established and the basic survey was done in spring of 1988, studies of liming effects were performed in the period 1987-2002. On particular plots, 0, 1,308, 2,826, 3,924 and 8,478 kg/ha of fine limestone (grain size under 1 mm) was distributed by hands on the soil surface, as an application of 3 and 9 t/ha of "fine" and "coarse" material (together 5 variants including the control one). Results are available for the forestry practice, research has to continue in the future - potential of negative liming impacts is topical here, consisting in humus mineralization and nutrients losses. Maximum effects on the soil reaction were observed in 8-10 years after limestone application at the soil surface (F - horizon) and in the period 10-15 years lower (H, A - horizons). Base saturation dynamics shows the time delay 2-4 years following pH values. For the site protection, efficient forest stands are vitally important of pioneer as well as climax species.

Concentrations of air pollutants measured by passive samplers, atmospheric deposition to forest ecosystems, soil water chemistry, nutrient content in the soil and foliage were all measured within a study of the causes of forest decline in Nýdek Forest Range, Jablunkov Forest District (Silesian Beskids). Declining Norway spruce stands are situated in a region of historically high air pollution load, mainly from the Třinec and Ostrava agglomeration. Air pollution significantly decreased in the second half of the 1990s. Forest soils showing the insufficiency of main nutrients remain today as the main factor affecting the forest vitality. The situation in the soil is subsequently reflected in the nutrition level of the assimilation tissues. Simultaneous effects of other stressors (e.g. biotic, meteorological ones) can support a sudden worsening of the stand health and cause serious damage.

The paper summarizes the results of long-term measurements of soil temperature. The measurements were performed at two depths: 5 cm and 20 cm, on a series of five experimental plots with different stocking. The temperature of soil in the submontane beech forest at the Ecological Experimental Site (EES) Kremnické vrchy Mts. reached maximum values in August, minimum ones in February. The obtained data were processed using t-test and regression analysis - to evaluate the influence of soil temperature at the discussed soil depths on the biomass production in plants. An important influence of this factor was confirmed at both depths.

Height and diameter growth and volume production of 22 different clones of poplars were evaluated in Sliepkovce populetum at the age of 12 years. The populetum is situated on medium-heavy and medium humic uninundated alluvia of the Laborec river; typologically, the group of forest types is Ulmeto-Fraxinetum populeum. The results of evaluation documented that among the poplars under study the clones Gigant, OP-229 and BL achieved the best growth and maximum volume production while their mean height amounted to 22.9-23.8 m, mean diameter to 31.9-33.2 cm and average annual volume increment was 25.1-28.3 m³ per 1 ha. The growth of P. Rochester 20/66 poplar was considerably much slower: its mean height amounted to 17.3 m, mean diameter to 18.7 cm and average annual volume increment was 6.3 m³ per 1 ha. It will be necessary to carry out further studies to obtain more detailed values for these clones.

Large areas were deforested as a consequence of the immission calamity in the Czech Republic in the last decades. As a part of restoration activities, preparatory species were utilized, both planted and sown, to cover forest soils, to prevent soil erosion and to regenerate forest microclimate, necessary for reintroduction of target, climax species. Birch (especially Betula pendula Roth.) was among the mostly common preparatory species. Presented paper documents the effects of birch in the case of its cultivation on an intact soil. In this case, birch was documented as a species suitable in a short-time perspective, improving soil characteristics and forming effective shelter against increased humus mineralization.

Cultures in vitro made in agar are rather precarious, because gel strength varies both with the medium formula used and the source and grade of agar. Any solidifying agent (like for example agar) should be strong enough to support cultivated plantlets, yet liquid enough to allow the nutrients and drossy products from plants through the medium. It should also be a chemically inert material. Agar, especially in acid solutions, is an undefined constituent of culture media, namely in the mentioned properties. Silica sand, used in cultures of herbs up to the present time, is applicable also in cultures of Sorbus sudetica. The required acid medium is exactly defined if sand is substituted for agar. Similar cultures of wood species, including conifers, will be realized in future research.

Manipulated experiments with males of Ips typographus (L., 1758) were conducted in spruce stands in north-western Slovakia. Some of trees were stressed by a lack of water caused by preparation of roofs under canopy. Inoculation experiments with bark beetles were conducted on such trees. According to results, the differences in attack rates between differently positioned trees on slope were not statistically significant (P = 0.389 for bottom and middle and P = 0.924 for bottom and top, and P = 0.530 for middle and top trees, t-test). Also the differences in attacks rate and the speed of entry holes preparation between more stressed and less-stressed trees were not statistically significant (P = 0.321, t-test). Thus the results of inoculation confirmed that low level of water stress does not lead necessarily to higher attack rate and (neither) faster speed of entry holes preparation. The obtained results are discussed.

The basic principle of the economic reform of state forests of the CzechRepublic after 1990 was to separate ma­nagement in forests from the implementation of production activities. A state enterprise Forests of the Czech Republic with its headquarters in Hradec Králové was charged to manage state forests. From the former 7 enterprises of state forests 78 joint-stock companies have been established that carry out silvicultural and logging activities in state forests and in forests of other owners under the conditions of competition environment and on the basis of contracts. The paper deals with the exact evaluation of partial and present results of the development of forestry economic reform, aimed at the study of the behaviour of transformed and privatised forest joint-stock companies.

The aim of this paper is to contribute to the knowledge of dynamics of species diversity, abundance, distribution of fruiting bodies and dominance of macromycetes in mycocoenosis of beech monocultures. The problems were studied in beech monocultures on three permanent research plots with various impacts of air pollutants generated by the aluminium plant in Žiar nad Hronom. Over the research period we determined 121 macromycete species and one species of imperfect fungus. We found relatively balanced values of abundance, fruiting body distribution and species dominance on all the examined plots. The species diversity in groups consisting of the most dominant species was practically the same on each plot. As for the ecotrophic requirements of individual macromycetes, we can conclude that the diversity of tree parasites decreased with decreasing pollutant load. We also found out relatively balanced numbers of lignicolous saprophytes and terrestrial saprophytes on each research plot. Air pollutants also influenced the species spectrum of ectomycorrhizal macromycetes negatively (only 6 species on the plot with highest pollution stress and 21 species on the plot with lowest pollution stress).

Expansion and invasion of plants indicate successful colonization and competitive abilities of species. There are fewer invasive and expansive woody plants than herbs. Main expansive (native species) trees and shrubs are Acer platanoides, Acer pseudoplatanus, Clematis vitalba, Crataegus sp. div., Fraxinus excelsior, Prunus spinosa, Rubus sp. div., Sambucus nigra. Main invasive (alien species) are Acer negundo, Ailanthus altissima, Amorpha fruticosa, Cytisus scoparius, Fraxinus pennsylvanica, Lycium barbarum, Mahonia aquifolium, Physocarpus opulifolius, Pinus strobus, Populus × canadensis, Prunus serotina, Quercus rubra, Rhus typhina, Robinia pseudoacacia, Symphoricarpos albus, Syringa vulgaris. Dominant characteristics of expansive and invasive species are dispersibility of seeds and capacity of vegetative propagation.

The paper widely introduces European experience in wild cherry breeding because it is the first one from a prepared series of articles aimed at wild cherry breeding in the Czech Republic. Beginnings of wild cherry breeding program for the Czech forestry are described. Plus trees were certified, seed orchards, clone archives, progeny and clonal tests were established. Clones were tested for fl owering of grafts in reproductive plantations. Progenies and clones were tested for growth parameters in progeny and clonal tests. Progenies and clones in every breeding plantation were tested for damage by aphids.

We evaluated the growth of Norway spruce (Picea abies [L.] Karst.) seedlings on peat and bark substrates, on their mixes and on their mixes with agroperlite. We examined the basic quantitative traits (seedling diameters and heights), the number of produced seedlings per unit area, main root lengths, number of axial shoots and dry weight of shoots and roots. Besides the study of substrate influence on the biometrical characteristics of seedlings applied standard fertilisation an experiment was established as the second variant where the effect of biostimulants was tested; they were supplied by the Jaminex Company. The experiment was established with three replications by standard technologies used in forest operations. A conclusion can be drawn that in our experiments peat was found to be the most suitable substrate for production of Norway spruce seedlings. Positive effects of biostimulants were highly significant on almost all tested substrate mixes.