Journal of Forest Science, 2022 (vol. 68), issue 5
Development of loop mediated isothermal amplification for rapid species detection of Armillaria ostoyae using assimilating probeOriginal Paper
Tomáš Tonka, Dagmar Stehlíková, Lucie Walterová, Vladislav Čurn
J. For. Sci., 2022, 68(5):163-169 | DOI: 10.17221/161/2021-JFS 
We introduced here the first loop mediated isothermal amplification (LAMP) assay for the identification of honey fungus, Armillaria ostoyae, a basidiomycote playing an important role in spruce declines in the Palaearctic region. In total, 101 isolates, representing three Armillaria species, were used to develop a new LAMP assay to determine species specific identification. We have here described LAMP primers enhanced with fluorescent dye that are able to amplify A. ostoyae DNA and detect fungi in a fast single step reaction. The detection limit of LAMP was 1 pg of genomic DNA per reaction. We optimized a new LAMP assay for...
Ectomycorrhiza-hydrogel additive enhanced growth of Norway spruce seedlings in a nutrient-poor peat substrateOriginal Paper
Ivan Repáč, Zuzana Parobeková, Martin Belko
J. For. Sci., 2022, 68(5):170-181 | DOI: 10.17221/29/2022-JFS
Seedling quality is an important input affecting the outplanted seedling performance. Morphological attributes and association with symbiotic ectomycorrhizal (ECM) fungi belong to influential traits determining seedling quality. In this study, the effect of pre-sowing applied commercial additives Ectovit (containing hydrogel and ECM fungi), Mycorrhizaroots (ECM fungi and nutrients) and Vetozen (natural mineral fertilizer) on the growth and ectomycorrhiza formation of Norway spruce seedlings grown in nutrient-poor pure peat in an open nursery site was assessed. Two-year-old bareroot seedlings were transplanted into containers. No significant growth...
Cut slope stability assessment along forest roads using the limit equilibrium approaches and Slide softwareOriginal Paper
Mehran Nasiri, Mojtaba Mohammadzade
J. For. Sci., 2022, 68(5):182-189 | DOI: 10.17221/21/2022-JFS
Calculating the factor of safety (FS) as slope stability factor is necessary to prevent environmental damage. Therefore, this paper aims to (i) calculate FS using the limit equilibrium approaches and Slide software and (ii) investigate the stability of slopes according to calculated FS (Janbu and Bishop methods) and status of different sites in the study area (Hyrcanian forest of Iran). Six landslides were selected along the forest road by a field survey. Landslide dimensions including length, width, and height were measured using meters. Slope gradients were measured using the Suunto clinometer. The Slide...
Prediction of flood discharge and flood flow depth using a hydraulic model and flood marks on the trees in ungauged forested watershedsOriginal Paper
Vahid Gholami
J. For. Sci., 2022, 68(5):190-198 | DOI: 10.17221/6/2022-JFS
It is difficult to estimate flood discharges and the flood zones as well as to design hydraulic structures in rivers without using hydrometric stations. Furthermore, using different models to determine the mentioned cases will be accompanied by errors. Therefore, flood marks on the trunks of trees located in the Babolrood riverbed were used to determine the peak discharge, flood flow depth, and flood zone in northern Iran. First, a hydraulic model for the study river was provided using topographic maps with a scale of 1: 1 000, HEC-GeoRAS extension (GIS), and HEC-RAS model. Then, the flood marks of past floods in the form of silt and clay sediments...