J. For. Sci., 2023, 69(4):144-157 | DOI: 10.17221/11/2023-JFS

Distribution of genetic variability in mature and progeny populations of Abies alba Mill. from the Polish Western and Eastern CarpathiansOriginal Paper

Marcin Zarek ORCID...1, Marta Kempf ORCID...2
1 Department of Forest Ecosystem Protection, Faculty of Forestry, University of Agriculture in Krakow, Poland
2 Department of Ecology and Silviculture, Faculty of Forestry, University of Agriculture in Krakow, Poland

This study aimed to compare patterns in the genetic structure of 27 mature stands and the natural regeneration of A. alba in the Eastern and Western Carpathians within the introgression zone of two refugial lineages from the Apennine and Balkan peninsulas. The distribution of the genetic diversity of fir stands was analysed using paternally inherited chloroplast DNA and five nuclear dominant inter simple sequence repeat markers (ISSRs). The study showed that the Balkan haplotype prevailed in both parental and progeny populations, and this haplotype was found in many mature Eastern Carpathian stands. Relatively high levels of genetic diversity were found in the mature stands (effective number of alleles Ne = 1.517, Shannon index I = 0.436, expected heterozygosity He = 0.295) and progeny (Ne = 1.515, I = 0.436 and He = 0.294) of silver fir. The analysis of molecular variance (AMOVA) revealed slight differences among the mature fir stands from the Western and Eastern Carpathians, with a value of 1.1%. According to principal coordinates analysis (PCoA) and STRUCTURE analyses, the populations, including stands of mature and progeny trees, were genetically separated into two groups. Slight genetic differences between the mature and progeny populations in the Polish Carpathians indicate sound gene pool transmission, which is essential for creating new selection and breeding programmes.

Keywords: chloroplast DNA; genetic diversity; genetic lineages; silver fir; simple sequence repeat markers (ISSR)

Received: January 25, 2023; Revised: March 3, 2023; Accepted: March 7, 2023; Prepublished online: April 4, 2023; Published: April 25, 2023  Show citation

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Zarek M, Kempf M. Distribution of genetic variability in mature and progeny populations of Abies alba Mill. from the Polish Western and Eastern Carpathians. J. For. Sci. 2023;69(4):144-157. doi: 10.17221/11/2023-JFS.
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