J. For. Sci., 2023, 69(3):124-126 | DOI: 10.17221/189/2022-JFS

Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham.Short Communication

Arturo Alonso Armas Silva ORCID...1, Lourdes Georgina Iglesias Andreu ORCID...1, Marco Antonio Ramírez Mosqueda2
1 Institute of Biotechnology and Applied Ecology (INBIOTECA), Veracruz University, Veracruz, Mexico
2 Faculty of Biological and Agricultural Sciences, Veracruz University, Veracruz, Mexico


The objective of the present work was to evaluate the efficacy of use of the RITA® temporary immersion system in the large-scale propagation of P. patula. The effects of four concentrations (0.00 µM, 4.50 µM, 9.00 µM, and 13.51 µM) of 6-benzylaminopurine (BAP) on 10 hypocotyl explants were studied using a completely randomised design with three replicates per treatment. Five hypocotyl explants were grown in 250 mL RITA® containers of Woody Plant Culture Medium (WPM) supplemented with 20 g·L–1 sucrose and 10 mg·L–1 vitamins from Murashige and Skoog (MS) culture medium. The frequency of immersion of the explants into the culture medium was 2 min every 8 hours. The number of adventitious buds and calli formed, as well as shoot growth, were evaluated after 6 weeks of in vitro culture. The 4.50 µM concentration of BAP was the best treatment for shoot production (5 shoots per plant) and shoot length (1.32 cm). These results could help the widespread vegetative propagation of this important forest species.

Keywords: conifers; in vitro; micropropagation; temporary immersion system; recipient for automated temporary immersion

Received: December 16, 2022; Accepted: March 7, 2023; Published: March 20, 2023  Show citation

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Alonso Armas Silva A, Iglesias Andreu LG, Ramírez Mosqueda MA. Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham. J. For. Sci. 2023;69(3):124-126. doi: 10.17221/189/2022-JFS.
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