J. For. Sci., 2021, 67(9):420-426
Identification and genetic diversity of Acer ibericum (Aceraceae) in South CaucasusOriginal Paper
- 1 Department of Botany and Molecular Evolution, Senckenberg Research Institute and Natural History Museum, Frankfurt/Main, Germany
- 2 Agroforestry Research Division, Scientific-Research Center of Agriculture, Tbilisi, Georgia
- 3 Department of Phytology, Faculty of Forestry, Technical University, Zvolen, Slovakia
- 4 Bioinformatics Core, Scientific-Research Center of Agriculture, Tbilisi, Georgia
- 5 Faculty of Agrarian and Natural Sciences, Agricultural University of Georgia, Tbilisi, Georgia
Internal transcribed spacer (ITS) sequence was tested in Acer ibericum for its suitability as a barcoding as well as population genetic marker. The marker was reliably used to identify A. ibericum as well as to gain preliminary insights into the genetic diversity of Georgian populations. MegaBLAST searches of studied samples against the GenBank database revealed that all studied accessions could be considered Acer ibericum. Populations from Vashlovani National Park revealed the presence of unique ribotypes, and together with the population from Shavi Mountain, they were identified as the most genetically diverse ones. Based on presented data the populations from Eastern Georgia should be prioritized if conservation measures are planned.
Keywords: barcoding; genetic diversity; Georgia; internal transcribed spacer (ITS); maple
Published: September 15, 2021 Show citation
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References
- Akhalkatsi M (2010): Habitats of Georgia. Tbilisi, Natura 2000: 118.
- Calonje M., Martín-Bravo S., Dobeš C., Gong W., JordonThaden I., Kiefer C., Kiefer M., Paule J., Schmickl R., Koch M.A. (2009): Non-coding nuclear DNA markers in phylogenetic reconstruction. Plant Systematics and Evolution, 282: 257-280.
Go to original source...
- Chen S., Yao H., Han J., Liu C., Song J., Shi L., Zhu Y., Ma X., Gao T., Pang X., Luo K., Li Y., Li X., Jia X., Lin Y., Leon C. (2010): Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One, 5: e8613.
Go to original source...
Go to PubMed...
- Clement M., Snell Q., Walke P., Posada D., Crandall K. (2002): TCS: Estimating gene genealogies. In: Proceedings 16th International Parallel and Distributed Processing Symposium (IPDPS 2002), Fort Lauderdale, Apr 15-19, 2001: 184.
Go to original source...
- de Jong P.C. (1994): Taxonomy and reproductive biology of maples. In: van Gelderen D.M., de Jong P.C., Oterdoom H.J. (eds): Maples of the World. Portland, Timber Press: 69-103.
- Dolukhanov A. (1948): Two maples new for the Caucasus. Bulletin of Georgian Academy of Sciences, 9: 603-605. (in Georgian)
- Fazekas A.J., Kesanakurti P.R., Burgess K.S., Percy D.M., Graham S.W., Barrett S.C.H., Newmaster S.G., Hajibabaei M., Husband B.C. (2009): Are plant species inherently harder to discriminate than animal species using DNA barcoding markers? Molecular Ecology Resources, 9: 130-139.
Go to original source...
Go to PubMed...
- Grimm G.W., Denk T. (2014): The Colchic region as refuge for relict tree lineages: Cryptic speciation in field maples. Turkish Journal of Botany, 38: 1050-1066.
Go to original source...
- Grimm G.W., Renner S.S., Stamatakis A., Hemleben V. (2006): A nuclear ribosomal DNA phylogeny of Acer inferred with maximum likelihood, splits graphs, and motif analysis of 606 sequences. Evolutionary Bioinformatics Online, 2: 7-22.
Go to original source...
- Grimm G.W., Denk T., Hemleben V. (2007): Evolutionary history and systematics of Acer section Acer - a case study of low-level phylogenetics. Plant Systematics and Evolution, 267: 215-253.
Go to original source...
- Han Y.-W., Duan D., Ma X.-F., Jia Y., Liu Z.-L., Zhao G.-F., Li Z.-H. (2016): Efficient identification of the forest tree species in Aceraceae using DNA barcodes. Frontiers in Plant Science, 7: 1707.
Go to original source...
Go to PubMed...
- Hebert P.D.N., Cywinska A., Ball S.L., deWaard J.R. (2003): Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270: 313-321.
Go to original source...
Go to PubMed...
- Johnson L.P.V. (1939): A descriptive list of natural and artificial interspecific hybrids in North American forest-tree genera. Canadian Journal of Research, 17: 411-444.
Go to original source...
- Katoh K., Standley D.M. (2013): MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30: 772-780.
Go to original source...
Go to PubMed...
- Kearse M., Moir R., Wilson A., Stones-Havas S., Cheung M., Sturrock S., Buxton S., Cooper A., Markowitz S., Duran C., Thierer T., Ashton B., Meintjes P., Drummond A. (2012): Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28: 1647-1649.
Go to original source...
Go to PubMed...
- Khademi H., Mehregan I., Assadi M., Nejadsatari T., Zarre S. (2016): Molecular phylogeny of Acer monspessulanum L. subspecies from Iran inferred using the ITS region of nuclear ribosomal DNA. Biodiversitas Journal of Biological Diversity, 17: 16-23.
Go to original source...
- Lachashvili J., Lachashvili N., Khachidze M. (2007): Conspectus of Flora of Kiziki (Eastern Georgia). Tbilisi, Universal: 375. (in Georgian)
- Leigh J.W., Bryant D. (2015): POPART: full-feature software for haplotype network construction. Methods in Ecology and Evolution, 6: 1110-1116.
Go to original source...
- Librado P., Rozas J. (2009): DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinfomatics, 25: 1451-1452.
Go to original source...
Go to PubMed...
- Marschall von Bieberstein F.A. (1808): Flora Taurico-Caucasica exhibens stirpes phaenogamas, in Chersoneso Taurica et regionibus caucasicis sponte crescentes. Charkovia: 478. (in Latin)
Go to original source...
- Nei M. (1987): Molecular Evolutionary Genetics. New York, Columbia University Press: 512.
Go to original source...
- Nei M., Tajima F. (1983): Maximum likelihood estimation of the number of nucleotide substitutions for restriction sites data. Genetics, 105: 207-217.
Go to original source...
Go to PubMed...
- Pojárkova A.J. (1949): Aceraceae Lindl. In: Komarov V.A. (ed.): Flora SSSR 14. Moskva, Leningrad, Izd. Akademii Nauk SSSR: 580-622. (in Russian)
- Ratnasingham S., Hebert P.D.N. (2013): A DNA-based registry for all animal species: the Barcode Index Number (BIN) system. PLoS One, 8: e66213.
Go to original source...
Go to PubMed...
- Tarkhnishvili D., Chaladze G. (eds) (2013): Georgian biodiversity database. Available at: http://www.biodiversitygeorgia.net (Accessed 7 December 2020).
- van Gelderen D.M., de Jong P.C., Oterdoom H.J., van Hoey Smith J.R.P., Dudley T.R. (1994): Maples of the World. Portland, Timber Press: 458.
- WFO (2020): World Flora Online. Available at: http://www.worldfloraonline.org (Accessed 7 December 2020).
- Yaltirik F. (1967a): Contribution to the taxonomy of woody plants in Turkey. Notes from the Royal Botanical Garden Edinburgh, 28: 9-10.
- Yaltirik F. (1965b): Acer L. In: Davis P.H., Cullen J., Coode M.J.E. (eds): Flora of Turkey and the East Aegean Islands. Volume 2. Edinburgh, Edinburgh University Press: 509-519.
- Zhang Z., Schwartz S., Wagner L., Miller W. (2000): A greedy algorithm for aligning DNA sequences. Journal of Computational Biology, 7: 203- 214.
Go to original source...
Go to PubMed...
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