J. For. Sci., 2017, 63(2):53-61 | DOI: 10.17221/88/2016-JFS

Evaluation of the genetic diversity of selected Fagus sylvatica L. populations in the Czech Republic using nuclear microsatellitesOriginal Paper

Helena CVRČKOVÁ*, Pavlína MÁCHOVÁ, Lucie POLÁKOVÁ, Olga TRČKOVÁ
Forestry and Game Management Research Institute, Jíloviště-Strnady, Czech Republic

Fagus sylvatica Linnaeus (European beech), the ecologically and economically most important broadleaved tree species in the Czech Republic, was strongly reduced in the past. Today there are efforts to increase the proportion of beech to ensure optimal forest tree species composition. When extensively reintroducing beech, it is important to acquire more detailed knowledge of genetic diversity. Thirteen important beech populations in different stands in the territory of the Czech Republic were genotyped using 12 polymorphic nuclear microsatellite markers. The genotypic data from adult trees imply genetic differences between the populations. The estimated genetic diversity expressed as Shannon's information index ranged from 1.73 to 1.92. Thirteen beech populations showed excess homozygotes, as indicated by positive fixation index (F) values (F = 0.005-0.115). The pairwise FST values indicated low genetic differentiation between the 13 Czech beech populations, because they were greater than zero, that means they confirmed the presence of population structuring in Czech European beech. Not significant linear correlations were observed between genetic and geographic distances of the 13 beech populations studied on the basis of microsatellite markers. Twelve microsatellite markers were verified as highly polymorphic and suitable for genotyping of European beech populations.

Keywords: DNA analysis; European beech populations; gene reserves; genetic distance; genetic variability; simple sequence repeats

Published: February 28, 2017  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
CVRČKOVÁ H, MÁCHOVÁ P, POLÁKOVÁ L, TRČKOVÁ O. Evaluation of the genetic diversity of selected Fagus sylvatica L. populations in the Czech Republic using nuclear microsatellites. J. For. Sci. 2017;63(2):53-61. doi: 10.17221/88/2016-JFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Asuka Y., Tani N., Tsumura Y., Tomaru N. (2004): Development and characterization of microsatellite markers for Fagus crenata Blume. Molecular Ecology Notes, 4: 101-103. Go to original source...
    2. Buiteveld J., Vendramin G.G., Leonardi S., Kamer K., Geburek T. (2007): Genetic diversity and differentiation in European beech (Fagus sylvatica L.) stands varying in management history. Forest Ecology and Management, 247: 98-106. Go to original source...
    3. Čáp J., Novotný P., Cvrčková H., Máchová P., Fulín M., Frýdl J., Dostál J., Buriánek V., Beran F., Lefnar R., Poláková L., Malá J. (2015): Genetická charakterizace významných regionálních populací smrku ztepilého v České republice. Specializovaná mapa s odborným obsahem. JílovištěStrnady, VÚLHM: 32.
    4. Chybicki I.J., Trojankiewicz M., Oleksa A., Dzialuk A., Burczyk J. (2009): Isolation-by-distance within naturally established populations of European beech (Fagus sylvatica). Botany, 87: 791-798. Go to original source...
    5. Comps B., Gömöry D., Letouzy J., Thiébaut B., Petit R.J. (2001): Diverging trends between heterozygosity and allelic richness during postglacial colonization in the European beech. Genetics, 157: 389-397. Go to original source... Go to PubMed...
    6. Dounavi A., Koutsias N., Ziehe M., Hattemer H.H. (2010): Spatial patterns and genetic structures within beech populations (Fagus sylvatica L.) of forked and non-forked individuals. European Journal of Forest Research, 129: 1191-1202. Go to original source...
    7. Fulín M., Čáp J., Cvrčková H., Novotný P., Máchová P., Dostál J., Frýdl J., Beran F. (2016): Genetická charakterizace významných regionálních populací jedle bělokoré v České republice. Specializovaná mapa s odborným obsahem. Jíloviště-Strnady, VÚLHM: 42.
    8. Gömöry D., Hynek V., Paule L. (1998): Delineation of seed zones for European beech (Fagus sylvatica L.) in the Czech Republic based on isozyme gene markers. Annals of Forest Science, 55: 425-436. Go to original source...
    9. Hasenkamp N., Ziegenhagen B., Mengel C., Schulze L., Schmitt H.P., Liepelt S. (2011): Towards a DNA marker assisted seed source identification: A pilot study in European beech (Fagus sylvatica L.). European Journal of Forest Research, 130: 513-519. Go to original source...
    10. Hintze J.L. (2012): NCSS 8. Kaysville, NCSS: 2823.
    11. Jump A.S., Peñuelas J. (2007): Extensive spatial genetic structure revealed by AFLP but not SSR molecular markers in the wind-pollinated tree, Fagus sylvatica. Molecular Ecology, 16: 925-936. Go to original source... Go to PubMed...
    12. Kalinowski S.T., Taper M.L., Marshall T.C. (2007): Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology, 16: 1099-1106. Go to original source... Go to PubMed...
    13. Konnert M., Ziehe M., Trober U., Mauer W., Janßen A., Sander T., Hussendörfer E., Hertel H. (2000): Genetische Variation der Buch (Fagus sylvatica L.) in Deutschland: Gemeinsame Auswertung genetischer Inventuren über verschiedene Bundesländer. Forts und Holz, 55: 403-408.
    14. Lefèvre S., Wagner S., Petit R.J., de Lafontaine G. (2012): Multiplexed microsatellite markers for genetic studies of beech. Molecular Ecology Resources, 12: 484-491. Go to original source... Go to PubMed...
    15. Meloun M., Militký J. (2011): Statistical Data Analysis, A Practical Guide with 1250 Exercises and Answer Key on CD. New Delhi, Woodhead Publishing India: 1600. Go to original source...
    16. Ministry of Agriculture of the Czech Republic (2015): Information on forests and forestry in the Czech Republic by 2014. Available at http://eagri.cz/public/web/file/433136/ZZ2014AJ_16112015.pdf (accessed June 27, 2016).
    17. Nei M. (1972): Genetic distance between populations. American Naturalist, 106: 283-392. Go to original source...
    18. Oddou-Muratorio S., Klein E.K., Vendramin G.G., Fady B. (2011): Spatial vs. temporal effects on demographic and genetic structures: The roles of dispersal, masting and differential mortality on patterns of recruitment in Fagus sylvatica. Molecular Ecology, 20: 1997-2010. Go to original source... Go to PubMed...
    19. Pastorelli R., Smulders M.J.M., Van't Westende W.P.C., Vosman B., Giannini R., Vettori C., Vendramin G.G. (2003): Characterization of microsatellite markers in Fagus sylvatica L. and Fagus orientalis Lipsky. Molecular Ecology, 3: 76-78. Go to original source...
    20. Peakall R., Smouse P.E. (2012): GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformatics, 28: 2537-2539. Go to original source... Go to PubMed...
    21. Piotti A., Leonardi S., Buiteveld J., Geburek T., Gerber S., Kramer K., Vettori C., Vendramin G.G. (2012): Comparison of pollen gene flow among four European beech (Fagus sylvatica L.) populations characterized by different management regimes. Heredity, 108: 322-331. Go to original source... Go to PubMed...
    22. Pluess A.R., Määttänen K. (2013): Characterization of eighteen novel microsatellite markers and multiplex PCR protocol for Fagus sylvatica. Conservation Genetics Resources, 5: 311-314. Go to original source...
    23. Scalfi M., Troggio M., Piovani P., Leonardi S., Magnaschi G., Vendramin G.G., Menozzi P. (2004): A RAPD, AFLP and SSR linkage map, and QTL analysis in European beech (Fagus sylvatica L.). Theoretical and Applied Genetics, 108: 433-441. Go to original source... Go to PubMed...
    24. Seifert S., Vornam B., Finkeldey R. (2012): DNA sequence variation and development of SNP markers in beech (Fagus sylvatica L.). European Journal of Forest Research, 131: 1761-1770. Go to original source...
    25. Svoboda P. (1955): Lesní dřeviny a jejich porosty. Část II. Prague, Státní zemědělské nakladatelství v Praze: 573.
    26. Tanaka K., Tsumura Y., Nakamura T. (1999): Development and polymorphism of microsatellite for Fagus crenata and the closely related species, F. japonica. Theoretical and Applied Genetics, 99: 11-15. Go to original source...
    27. Úradníček L., Maděra P., Tichá S., Koblížek J. (2009): Dřeviny České republiky. Kostelec nad Černými lesy, Lesnická práce, s.r.o.: 366.
    28. Van Oosterhout C.V., Hutchinson W.F., Wills D.P.M., Shipley P. (2004): Micro-Checker: Software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology, 4: 535-538. Go to original source...
    29. Vettori C., Vendramin G.G., Anzidei M., Pastorelli R., Paffetti D., Giannini R. (2004): Geographic distribution of chloroplast variation in Italian populations of beech (Fagus sylvatica L.). Theoretical and Applied Genetics, 109: 1-9. Go to original source... Go to PubMed...
    30. Vornam B., Decarli N., Gailing O. (2004): Spatial distribution of genetic variation in a natural beech stand (Fagus sylvatica L.) based on microsatellite markers. Conservation Genetics, 5: 561-570. Go to original source...
    31. Wright S. (1965): The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution, 19: 395-420. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content