J. For. Sci., 2016, 62(5):198-203 | DOI: 10.17221/82/2015-JFS

In vitro regeneration of Pistacia vera L. from nodal explantsOriginal Paper

B. Benmahioul1, M. Kaïd-Harche2, F. Daguin3
1 Department of Forest Resources, Faculty of Natural Sciences and Life, Earth Sciences and the Universe, University of Abou-Bekr Belkaïd Tlemcen, Tlemcen, Algeria
2 Department of Biotechnology, Faculty of Sciences, University of Sciences and Technology of Oran Mohamed Boudiaf, Oran, Algeria
3 Research Institute of Horticulture and Seeds, University of Angers, Angers, France

To enhance the induction of shoots, the excised nodes were cultured on Murashige and Skoog medium containing different cytokinins and various concentrations. The best conditions for shoot induction and growth were with 6-benzyladenine at 1.0 mg.l-1. For axillary shoot proliferation, the excellent result was obtained using 2.0 mg.l-1 meta-topoline. Well-developed shoots (more than 2 cm in length) were successfully rooted ex vitro at 82% by treatment with commercial rooting powder (2% indole-3-butyric acid; Rhizopon®). Rooted plantlets were acclimatized in the greenhouse and the survival rate of transplantation reached 80%.

Keywords: sterilizing agents; morphogenic response; cytokinin; shoot proliferation; ex vitro rooting; tissue culture

Published: May 31, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Benmahioul B, Kaïd-Harche M, Daguin F. In vitro regeneration of Pistacia vera L. from nodal explants. J. For. Sci. 2016;62(5):198-203. doi: 10.17221/82/2015-JFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abousalim A., El Mahboul B., Walali L.D. (1991): La multiplication in vitro de Pistacia vera L. Revue Réseau Amélioration de la Production Agricole du Milieu Aride, 3: 73-79.
    2. Ahmed G.U., Shin S.L., Lee C.H. (2011): In vitro culture responses of Cratoneuron decipiens (Brid.) G. Roth gametophyte for micropropagation. Horticulture, Environment and Biotechnology, 52: 614-620. Go to original source...
    3. Barghchi M., Alderson P.G. (1983): In vitro propagation of Pistacia vera L. from seedling tissues. Journal of Horticultural Sciences, 58: 435-445. Go to original source...
    4. Barghchi M., Alderson P.G. (1989): Pistachio (Pistacia vera L.). In: Bajaj Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Berlin, Springer-Verlag: 68-98. Go to original source...
    5. Benmahioul B. (2009): Amélioration de la micropropagation in vitro du pistachier (Pistacia vera L.) en vue de l'extension des vergers en Algérie. [Ph.D. Thesis.] Oran, University of Sciences and Technology of Oran Mohamed Boudiaf: 129.
    6. Benmahioul B., Kaid-Harche M., Dorion N., Daguin F. (2009): In vitro embryo germination and proliferation of pistachio (Pistacia vera L.). Scientia Horticulturae, 122: 479-483. Go to original source...
    7. Benmahioul B., Dorion N., Kaid-Harche M., Daguin F. (2012a): Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.). Plant Cell Tissue and Organ Culture, 108: 353-358. Go to original source...
    8. Benmahioul B., Kaid-Harche M., Daguin F. (2012b): Influence of activated charcoal on in vitro germination and growth of plantlets of pistachio (Pistacia vera L.). International Journal of Science and Nature, 3: 613-616.
    9. Benmahioul B., Kaid-Harche M., Daguin F. (2015): Cryopreservation of Pistacia vera embryonic axes. Journal of Forest Science, 61: 182-187. Go to original source...
    10. Bonga J.M., Von Aderkas P. (1992): In Vitro Culture of Trees. Dordrecht, Kluwer Academic Publishers: 236. Go to original source...
    11. Bozorgi M., Memariani Z., Mobli M., Surmaghi M.H.S., Shams-Ardekani M.R., Rahimi R. (2013): Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): A review of their traditional uses, phytochemistry, and pharmacology. The Scientific World Journal, 2013: 33. Go to original source... Go to PubMed...
    12. Can C., Ozaslan M., Toremen H., Sarpkaya K., Iskender E. (2006): In vitro micrografting of pistachio, Pistacia vera L. var. Siirt, on wild pistachio rootstocks. Journal of Cell and Molecular Biology, 5: 25-31.
    13. Chatibi A., Kchouk M.E., Ben Abdallah F., Zemni H., Ghorbel A. (1995): Rooting improvement of Pistacia vera L. cv. Mateur by in vitro culture of apices and cuttings. Acta Horticulturae, 419: 213-220. Go to original source...
    14. Danso K.E., Azu E., Elegba W., Asumeng A., Amoatey H.M., Klu G.Y.P. (2011): Effective decontamination and subsequent plantlet regeneration of sugarcane (Sacchrum officinarum L.) in vitro. International Journal of Integrative Biology, 11: 90-96.
    15. Gamborg O.L., Miller R.A., Ojima K. (1968): Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151-158. Go to original source... Go to PubMed...
    16. George E.F. (1993): Plant Propagation by Tissue Culture. Part I. The Technology. 2nd Ed. Edington, Exegetics: 574.
    17. Holtz B., Ferguson L., Allen G.E. (1995): Rootstock production and budding. In: Ferguson L. (ed.): Pistachio Production. Davis, University of California: 54-56.
    18. Huetteman C.A., Preece J.E. (1993): Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell Tissue and Organ Culture, 33: 105-119. Go to original source...
    19. Husain M.K., Anis M. (2009): Rapid in vitro multiplication of Melia azedarach L. (a multipurpose woody tree). Acta Physiologiae Plantarum, 31: 765-772. Go to original source...
    20. Jacquy P. (1973): La culture du pistachier en Tunisie. Projet de vulgarisation de la culture du pistachier. Tunis, INRAT: 97.
    21. Joley L.L.E. (1979): Pistachios. In: Jaynes R.A. (ed.): Nut Tree Culture in North America. Hamden, Northern Nut Growers Association, Inc.: 163-174.
    22. Kilinç F.M.¸ Süzerer V., Özden Çiftçi Y., Onay A., Yildirim H., Uncuoğlu A.A., Tilkat E., Koç I., Akdemir O.F., Metin O.K. (2015): Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers. Plant Growth Regulation, 75: 75-88. Go to original source...
    23. Letham D.S., Palni L.M.S. (1983): The biosynthesis and metabolism of cytokinins. Annual Review of Plant Physiology, 34: 163-197. Go to original source...
    24. Maggs D.H. (1975): Prospects for pistachio nut growing in Australia. West Australian Nutgrowing Society, 1: 47-63.
    25. Martin K.P. (2003): Rapid axillary bud proliferation and ex vitro rooting of Eupatorium triplinerve. Biologia Plantarum, 47: 589-591. Go to original source...
    26. Mascarello C., Fascella G., Zizzo G.V., Mantovani E., Ruffoni B. (2007): In vivo and in vitro propagation of Pistacia lentiscus L. Acta Horticulturae, 764: 299-306. Go to original source...
    27. Meynier V. (1985): Propagation in vitro de noyers sélectionnés (Juglans regia, Juglans nigra × regia): Culture de noeuds, culture de méristèmes. [Ph.D. Thesis.] Nancy, University of Nancy I: 115.
    28. Murashige T., Skoog F. (1962): A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiologia Plantarum, 15: 473-479. Go to original source...
    29. Nin S., Schiff S., Bennici A., Magherini R. (1994): In vitro propagation of Artemisia absinthium L. Advances in Horticultural Science, 8: 145-147.
    30. Onay A. (2000): Micropropagation of pistachio from mature trees. Plant Cell Tissue and Organ Culture, 60: 159-162. Go to original source...
    31. Onay A. (2003): Micropropagation of pistachio. In: Jain S.M., Ishii K. (eds): Micropropagation Forest Trees and Fruits. Dordrecht, Kluwer Academic Publishers: 565-588. Go to original source...
    32. Onay A. (2005): Pistachio, Pistacia vera L. In: Jain S.M., Gupta P. (eds): Protocols for Somatic Embryogenesis in Woody Plants. Dordrecht, Kluwer Academic Publishers: 289-300. Go to original source...
    33. Ozden-Tokatli Y., Ozudogru E.A., Akcin A. (2005): In vitro response of pistachio nodal explants to silver nitrate. Scientia Horticulturae, 106: 415-426. Go to original source...
    34. Riffaud J.L., Cornu D. (1981): Utilisation de la culture in vitro pour la multiplication de merisiers adultes (Prunus avium L.) sélectionnés en forêt. Agronomie, 1: 633-640. Go to original source...
    35. Sen M.K., Hassan M.M., Nasrin S., Jamal M.A.H.M., MamunOr-Rashid A.N.M., Dash B.K. (2013): In vitro sterilization protocol for micropropagation of Achyranthes aspera L. node. International Research Journal of Biotechnology, 4: 89-93.
    36. Sujatha M., Reddy T.P. (1998): Differential cytokinin effects on the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis L.). Plant Cell Reports, 17: 561-566. Go to original source... Go to PubMed...
    37. Wie Q., Cao J., Qian W., Xu M., Li Z., Ding Y. (2015): Establishment of an efficient micropropagation and callus regeneration system from the axillary buds of Bambusa ventricosa. Plant Cell Tissue and Organ Culture, 122: 1-8. Go to original source...
    38. Xu J., Wang Y., Zhang Y., Chai T. (2008): Rapid in vitro multiplication and ex vitro rooting of Malus zumi (Matsumura) Rehd. Acta Physiologiae Plantarum, 30: 129-132. Go to original source...
    39. Yang Z., Lüdders P. (1993): In vitro propagation of pistachio (Pistacia vera L.). Gartenbauwissenschaft, 59: 30-34.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content