J. For. Sci., 2014, 60(2):70-74 | DOI: 10.17221/80/2013-JFS

Micropropagation of Shorea robusta: an economically important woody plantOriginal Paper

M. Singh, S. Sonkusale, Ch. Niratker, P. Shukla
Devleela Biotechs, Chhattisgarh, India

Shorea robusta is a valuable tree species which provides good quality timber along with other useful materials like seeds which can be used as a source of starch. Woody plants are difficult to regenerate under in vitro conditions and only some success has been achieved so far. Here we have presented the data for successful in vitro regeneration of S. robusta using nodal explants. Shoot proliferation and rooting were also successfully achieved in subsequent subcultures. The best medium for shoot initiation and proliferation was found to be WPM with 1.0 mg.l-1 BAP and 0.5 mg.l-1 NAA and 1.0 mg.l-1 BAP +0.5 mg.l-1 NAA, respectively. Likewise for rooting WPM medium with 0.5 mg.l-1 IBA was found to be the best medium.

Keywords: WPM medium; BAP medium; IBA medium

Published: February 28, 2014  Show citation

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Singh M, Sonkusale S, Niratker C, Shukla P. Micropropagation of Shorea robusta: an economically important woody plant. J. For. Sci. 2014;60(2):70-74. doi: 10.17221/80/2013-JFS.
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    References

    1. Aliou N., Mamadou S.D., Dame N., Yaye Kene G.D. (2006): In vitro regeneration of adult trees of Bambusa vulgaris. African Journal of Biotechnology, 5: 1245-1248.
    2. Amo-Marco J.B., Lledo M.D. (1996): In vitro propagation of Salix tarraconensis Pau ex Font Quer an endemic and threatened plant. In Vitro Cellular and Developmental Biology - Plant, 32: 42-46. Go to original source...
    3. Gamborg O.L., Miller A., Ojima K. (1968): Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151-158. Go to original source... Go to PubMed...
    4. Kumar S., Singh S. (2009): Micropropagation of Prosopis cineraria (L.) Druce - a multipurpose desert tree. Researcher, 1: 28-32.
    5. Lloyd G., McCown B. (1980): Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Proceedings of the International Plant Propagator's Society, 30: 421-427.
    6. Lyyra S., Lima A., Merkla S.A. (2006): In vitro regeneration of Salix nigra from adventitious shoots. Tree Physiology, 26: 969-975. Go to original source... Go to PubMed...
    7. Murashige T., Skoog F. (1962): A revised medium for rapid growth and bioassays with tobacco cultures. Physiologia Plantarum, 15: 473-497. Go to original source...
    8. Nakamura K. (2006): Micropropagation of Shorea roxburghii and Gmelina arborea by shoot-apex culture. In: Suzuki I., Ishii K., Sakurai S., Sasaki S. (eds): Plantation Technology in Tropical Forest Science. Tokyo, SpringerVerlag: 137-150. Go to original source...
    9. Nanda R.M., Das P., Rout G.R. (2004): In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker. Annals of Forest Science, 61: 381-386. Go to original source...
    10. Orwa C., Mutua A., Kindt R., Jamnadass R., Simons A. (2009): Agroforestree Database: a Tree Reference and Selection Guide Version 4.0. Avaialable at http://www.worldagroforestry.org/af/treedb
    11. Pijut P.M., Lawson S.S., Michler C.H. (2011): Biotechnological efforts for preserving and enhancing temperate hardwood tree biodiversity, health, and productivity. In Vitro Cellular and Developmental Biology - Plant, 47: 123-147. Go to original source...
    12. Rajore S., Batra A. (2005): Efficient Plant regeneration via Shoot tip explants in Jatropha curcas. Journal of Plant Biochemistry and Biotechnology, 14: 73-75. Go to original source...
    13. Rao C.S., Eganathan P., Anand A., Balakrishna Reddy T.P. (1998): Protocol for in vitro propagation of Excoecaria agallocha L., a medicinally important mangrove species. Plant Cell Reports, 17: 861-865. Go to original source... Go to PubMed...
    14. Rout G.R. (2004): Effect of cytokinin and auxin on micro propagation of Cleitoria ternatea L. Biological Letters, 41: 21-26.
    15. Rout G.R., Samantaray S., Das P. (2000): In vitro manipulation and propagation of medicinal plants. Biotechnology Advances Journal, 18: 91-120. Go to original source... Go to PubMed...
    16. Saxena C., Rout G.R., Das P. (1998): Micropropagation of Psoralea coryfolia, L. Journal of Medicinal and Aromatic Plant Sciences, 20: 15-18.
    17. Schenk R.V., Hildebrandt A.C. (1972): Medium and techniques for induction and growth of monocotyledons and dicotyledons plant cell cultures. Canadian Journal of Botany, 50: 199-204. Go to original source...
    18. Shukla S., Shukla S.K., Mishra S.K. (2012) In vitro regeneration of multipurpose medicinal tree Stereospermum suaveolens - factors controlling the in vitro generation. Journal of Biotechnology and Biomaterials, 13 (Special Issue): 1-3. Go to original source...
    19. Singh K.P., Kushwaha C.P. (2005): Paradox of leaf phenology: Shorea robusta is a semi-evergreen species in tropical dry deciduous forests in India. Current Science, 88: 1820-1824.
    20. Thakur R., Rao P.S., Bapat V.A. (1998): In vitro plant regeneration in Melia azedarach L. Plant Cell Reports, 18: 127-131. Go to original source...
    21. Vengadesan G., Ganapathi A., Anand R.P., Anbazhagan V.R. (2002): In vitro propagation of Acacia sinuata (Lour.) Merr. via cotyledonary nodes. Agroforestry Systems, 55: 9-15. Go to original source...
    22. Vengadesan G., Ganapathi A., Anand R.P., Selvaraj N. (2003): In vitro propagation of Acacia sinuate (Lour.) Merr. from nodal segments of a 10-year-old tree. In Vitro Cellular and Developmental Biology - Plant, 39: 409-414. Go to original source...

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