J. For. Sci., 2013, 59(10):377-387 | DOI: 10.17221/39/2013-JFS

Analysis of the genetic structure of a model Scots pine (Pinus sylvestris) seed orchard for development of management strategiesOriginal Paper

O. Ivanek1, Z. Procházková1, K. Matějka2
1 Forestry and Game Management Research Institute, Jíloviště-Strnady, Czech Republic
2 IDS, Prague, Czech Republic

Genetic structure, diversity and clonal homogeneity were determined on the basis of the isozyme gene markers in a model Scots pine seed orchard in the north-eastern part of the Czech Republic that originated from natural forest regions NFR 28 and 29 (Jeseníky Mts.) and the 2nd and 3rd forest altitudinal zones (FAZ). Clone and ramet crop variation for the 2009-2011 period was assessed. Comparing the loci measured also in two pine orchards in central Slovakia, a similar proportion of homogeneous clones but considerably lower expected heterozygosity were found out for most of the measured isozyme loci. Heterozygosity of the investigated orchard did not change considerably after the exclusion of alien and wrongly placed ramets. No difference in average cone production between clones originating from different NFR was observed. Verification of the clone identity of seed orchards managed in a certain way can be suggested with the subsequent removal of alien ramets.

Keywords: fructification; gene markers; isozymes; Monte-Carlo test; Rao coefficient; Shannon index

Published: October 31, 2013  Show citation

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Ivanek O, Procházková Z, Matějka K. Analysis of the genetic structure of a model Scots pine (Pinus sylvestris) seed orchard for development of management strategies. J. For. Sci. 2013;59(10):377-387. doi: 10.17221/39/2013-JFS.
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References

  1. Afif M., Messaoud C., Boulila A., Chograni H., Bejaout A., Rejeb M.N., Boussaid M. (2008): Genetic structure of Tunisian natural carob tree (Ceratonia siliqua L.) populations inferred from RAPD markers. Annals of Forest Science, 65: 710. Go to original source...
  2. Barnett J.P. (1996): How seed orchard culture affects seed quality: Experience with the southern pines. The Forestry Chronicle, 72: 469-473. Go to original source...
  3. Bergmann F., Hattemer H.H. (1995): Isozyme loci and their allelic variation in Pinus sylvestris L. and Pinus cembra L. Silvae Genetica, 44: 286-289.
  4. Colas F., Bettez M. (2004): The biggest problems or information needs with respect to tree seed for the province of Québec. News Bulletin, 40: 3-4.
  5. El-Kassaby Y.A., Rudin D., Yazdani R. (1989). Levels of autcrossing and contamination in two Pinus sylvestirs L. seed orchards in northern Sweden. Scandinavian Journal of Forest Research, 4: 41-49 Go to original source...
  6. Gömöry D., Bruchánik R., Paule L. (2000): Effective population number estimation of three Scots pine (Pinus sylvestris L.) seed orchards based on an integrated assessment of flowering, floral phenology, and seed orchard design. Forest Genetics, 7: 65-75.
  7. Gömöry D., Bruchánik R., Longauer R. (2003): Fertility variation and flowering asynchrony in Pinus sylvestris: consequences for the genetic structure in seed orchards. Forest Ecology and Management, 174: 117-126. Go to original source...
  8. Gömöry D., Paule L. (1993): Inferences on mating system and genetic composition of a seed crop in the European larch (Larix decidua Mill.). Journal of Genetics and Breeding, 46: 309-314.
  9. Hertel H. (1997): Biochemisch-genetische Untersuchungen bei Kiefer (Pinus sylvestris L.): Anleitung zur Trennmethodik und Auswertung der Zymogramme. Hamburg, Wiedebusch: 59.
  10. Ivanek O., Procházková Z. (2006): Identifikace roubovanců a klonů ve dvou semenných sadech modřínu opadavého (Larix decidua Mill.). [Grafts and clonal identification in two seed orchards of European Larch (Larix decidua Mill.).]. Zprávy lesnického výzkumu, 51: 38-43.
  11. Jurásek A. (2011): Stabilizace funkcí lesa v antropogenně narušených a měnících se podmínkách prostředí. [Stabilization of forest functions in anthropically disturbed and changing environmental conditions]. Opočno, VÚLHM [CD-ROM]: 69.
  12. Kang K.S., Harju A.M., Lindgren D., Nikkanen T., Almqvist C., Suh G.U. (2001): Variation in effective number of clones in seed orchards. New Forests, 21: 17-33. Go to original source...
  13. Kang K.S., Lindgren D., Mullin T.J. (2001b): Prediction of genetic gain and gene diversity in seed orchard crops under alternative management strategies. Theoretical and Applied Genetics, 103: 1099-1107. Go to original source...
  14. Lia F., Gan S.M., Weng Q., Zhao X., Huang S.N., Li M., Chen S.Y., Wang Q.H., Shi F.Q. (2008): RAPD and morphological diversity among four populations of the tropical tree species Paramichelia baillonii (Pierre) Hu in China. Forest Ecology and Management, 255: 1793-1801. Go to original source...
  15. Lindgren D., Prescher F. (2005): Optimal clone number for seed orchards with tested clones. Silvae Genetica, 54: 80-92 Go to original source...
  16. Matějka K. (2009): Nápověda k programu SeqAn. [Sequential data distribution analyse.] Available at http://www.infodatasys.cz/software/hlp_seqan/seqan.htm
  17. Matějka K. (2010): Program IsoEnz. Databáze isoenzymových analýz. [Isozyme analysis database.] Available at http://www.infodatasys.cz/software/hlp_isoenz/isoenz.htm
  18. McKeand S., Mullin T.J., Byram R., White T. (2003). Deployment of genetically-improved loblolly and slash pines in the south. Journal of Forestry, 101: 32-37. Go to original source...
  19. Olsson T., Lindgren D., Li B. (2001): Balancing genetic gain and relatedness in seed orchards. Silvae Genetica, 50: 222-227.
  20. Pasteur N., Pasteur G., Bonhomme F., Catalan J., Britton-Davidian J. (1988): Practical Isozyme Genetics - Ellis Horwood Series in Gene Technology. New York, Wiley & Sons: 215.
  21. Paule L., Gömöry D. (1992): Genetic processes in seed orchards as illustrated by European larch (Larix decidus Mill) and Scots pine (Pinus sylvestris L.). In: Proceedings of the International Conference on Phytotechnique and Forest Management in Present Ecological Conditions. Zvolen, Technical University in Zvolen: 109-114.
  22. Prescher F. (2007): Seed Orchards - Genetic Considerations on Function, Management and Seed Procurement. [Doctoral Thesis.] Acta Universitatis Agriculturae Sueciae, 2007: 75.
  23. Rao C.R. (1982): Diversity and dissimilarity coefficients: a unified approach. Theoretical Population Biology, 21: 24-43. Go to original source...
  24. Shannon C.E., Weaver W. (1949): The Mathematical Theory of Communication. Urbana, University of Illinois: 144.
  25. Šindelář J., Frýdl J. (2000): První výsledky ověřování potomstev modřínu opadavého z osiva produkovaného v semenných sadech. [First results of verification of European larch offsprings produced from seed orchards seeds.] Lesnická práce, 79: 546-549
  26. Stoehr M., O'Neill G., Hollefreund C., Yeanchuk A. (2005): Within and among family variation of orchard and wild-stand progeny of interior spruce in British Columbia. Tree Genetics and Genomes, 1: 64-68 Go to original source...
  27. Viewegh J., Kusbach A., Mikeska M. (2003): Czech forest ecosystem classification. Journal of Forest Science, 49: 85-93. Go to original source...
  28. Wasielewska M., Klemm M., Burczyk J. (2005) : Genetic diversity and mating system of Scots pine plus trees. Dendrobiology, 53: 57-62
  29. Wendel J.F., Weeden N.F. (1990): Visualisation and interpretation of plant isozymes. In: Soltis D.E., Soltis P.S. (eds): Isozymes in Plant Biology. London, Chapman and Hall: 46-72. Go to original source...
  30. Wennström U., Bergsten U., Nilsson J.E. (2007): Seedling establishment and growth after direct seeding with Pinus sylvestris: effects of seed type, seed origin, and seeding year. Silva Fennica, 41: 299-314 Go to original source...

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