J. For. Sci., 2011, 57(7):277-280 | DOI: 10.17221/40/2010-JFS

Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants

D. Pavingerová, J. Bříza, H. Niedermeierová, J. Vlasák
Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic

In conifers and other plants with long reproductive cycles, transformed embryogenic tissues can serve as a convenient source of plant material for the testing of insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic tissue was obtained after the transformation of somatic embryos of Norway spruce (Picea abies (L.) Karst.) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only in a suitable embryogenic cell line sensitive to Agrobacterium. Out of the nine embryogenic lines tested only one gave transgenic callus.

Keywords: Agrobacterium tumefaciens; genetic engineering; GUS activity; Picea abies (L.) Karst.

Published: July 31, 2011  Show citation

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Pavingerová D, Bříza J, Niedermeierová H, Vlasák J. Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants. J. For. Sci. 2011;57(7):277-280. doi: 10.17221/40/2010-JFS.
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