J. For. Sci., 2009, 55(11):526-531 | DOI: 10.17221/106/2008-JFS
The use of cotyledon proteins to assess the genetic diversity in sweet holm oak
- 1 Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Córdoba, Spain
- 2 Departamento de Ingeniería Agroforestal, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Córdoba, Córdoba, Spain
Sweet holm oak (Quercus ilex ssp. ballota Desf. Samp.) is an important broad-leaved tree spread in the Mediterranean basin. In Spain, few studies on the genetic variability of this species have been displayed. Storage seed proteins are a useful tool in the evaluation of the genetic variability of many species. The objective of this study was to analyze the usefulness of cotyledon proteins as markers of the genetic diversity in sweet holm oak. The evaluated populations were highly polymorphic for the glutelins, being detected up to 32 polymorphic bands with a wide distribution among all them. Considering all evaluated populations, about 35.8% of the total allelic variation was distributed among populations. This method of analysis of cotyledon storage proteins (glutelins) could be considered an additional tool for the evaluation of genetic diversity in this species.
Keywords: seed storage proteins; genetic resources; sweet holm oak
Published: November 30, 2009 Show citation
References
- ALVAREZ J.B., MUÑOZ-DIEZ C., MARTÍN-CUEVAS A., LÓPEZ S., MARTÍN L.M., 2003. Cotyledon storage proteins as markers of the genetic diversity in Castanea sativa Miller. Theoretical and Applied Genetics, 107: 730-735.
Go to original source...
Go to PubMed...
- CASTROVIEJO S., LAÍNZ M., LÓPEZ G., MONSERRAT P., MUÑOZ F., PAIVA J., VILLAR L., 1990. Flora Ibérica. Plantas vasculares de la Península Ibérica e Islas Baleares. Vol. 2. C.S.I.C. Madrid, Real Jardín Botánico.
- COLLADA C., CASADO R., BARBER D., FERNANDEZ DE CALEYA R., ARAGONCILLO C., 1986. Characterization of seed protein fractions from Castanea spp. Journal of Experimental Botany, 37: 1872-1878.
Go to original source...
- COLLADA C., CABALLERO R.G., CASADO R., ARAGONCILLO C., 1991. Seed storage proteins in Fagaceae: similarity between Castanea globulins and Quercus glutelins. Plant Science, 75: 145-154.
Go to original source...
- DANNE F., HAWKINS L.K., HUANG H., 1999. Genetic variation and population structure of Castanea pumila var. ozarkensis. Journal of the American Society for Horticultural Science, 124: 666-670.
Go to original source...
- FONSECA P.A., FERREIRA R.B., TEIXEIRA A.R., 1997. Seed proteins from Quercus suber. Journal of Agriculture and Food Chemistry, 45: 3443-3447.
Go to original source...
- GEPTS P., 1990. Genetic diversity of seed storage proteins in plants. In: BROWN A.H.D., CLEGG M.T., KAHLER A.L., WEIR B.S. (eds), Plant population genetics, breeding and genetic resources. Suderland, Sinauer Associates Inc. Publishers: 64-82.
- HUANG H., DANNE F., KUBISIAK T.L., 1998. Allozyme and RAPD analysis of the genetic diversity and geographic variation in wild populations of the American chestnut Castanea dentata (Fagaceae). American Journal of Botany, 85: 1013-1021.
Go to original source...
- JIMÉNEZ M.P., DÍAZ-FERNÁNDEZ P.M., IGLESIAS S., DE TUERO M., GIL L., 1996. Las regiones de procedencia de Quercus ilex L. en España. Madrid, ICONA.
- LAEMMLI U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
Go to original source...
Go to PubMed...
- MARSHALL D.R., BROWN A.H.D., 1975. Optimum sampling strategies in genetic conservation. In: FRANKEL O.H., HAWKES J.G. (eds), Crop genetic resources for today and tomorrow. Cambridge, Cambridge University Press: 53-70.
- MARTÍN M.A., MARTÍN L.M., ALVAREZ J.B., 2005. Cotyledon storage proteins in European sweet chestnut. Acta Horticulturae, 693: 459-463.
Go to original source...
- NEI M., 1973. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences, USA, 70: 3321-3323.
Go to original source...
Go to PubMed...
- VÁZQUEZ PARDO F.M., 1998. Semillas del género Quercus L. (Biología ecología y manejo). Consejería de Agricultura y Comercio. Junta de Extremadura, Badajoz.
- VILLANI F., PIGLIUCCI M., BENEDETTELLI S., CHERUBINI M., 1991. Genetic differentiation among Turkish chestnut (Castanea sativa Mill.) populations. Heredity, 66: 131-136.
Go to original source...
- WRIGLEY C., BEKES F., BUSHUK W. (eds), 2006. Gliadin and glutenin: the unique balance of wheat quality. St. Paul, AACC International Press.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.