J. For. Sci., 2009, 55(2):75-80 | DOI: 10.17221/84/2008-JFS

Polyamines during somatic embryo development in Norway spruce (Picea abies [L.])

J. Malá1, M. Cvikrová2, P. Máchová2, O. Martincová2
1 Forestry and Game Management Research Institute, Strnady, Czech Republic
2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic

Contents of free polyamines (putrescine, spermidine and spermine) were determined in different developmental stages of Norway spruce (Picea abies [L.] Karst.) somatic embryos by means of HPLC. Determinations were performed embryogenic tissue after 4 weeks of the growth on proliferation medium, after 2 and 5 weeks of the culturing on maturation medium, and 2 weeks after desiccation. Maturation of somatic embryos (after 5 weeks) was accompanied by increase of concentrations of putrescine (2.3 times) and spermidine (3.2 times). In comparison with above mentioned polyamines, spermine concentrations were significantly lower (4.3 times). Two weeks after desiccation, the concentrations of putrescine decreased 5.4 times and spermidine 2.2 times in comparison with mature embryos. To improve the efficiency of somatic embryogenesis of less responsive genotypes, the supplementation of growth media by polyamines is discussed.

Keywords: Norway spruce; somatic embryogenesis; putrescine; spermidine; spermine

Published: February 28, 2009  Show citation

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Malá J, Cvikrová M, Máchová P, Martincová O. Polyamines during somatic embryo development in Norway spruce (Picea abies [L.]). J. For. Sci. 2009;55(2):75-80. doi: 10.17221/84/2008-JFS.
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