J. For. Sci., 2004, 50(12):553-558 | DOI: 10.17221/4656-JFS

In vitro propagation of European larch (Larix decidua Mill.)

V. Chalupa
Faculty of Forestry and Environment, Czech University of Agriculture, Prague, Czech Republic

Methods of in vitro propagation of Larix decidua are described in the paper. The influence of explant source, tree age, genotype, composition of nutrient media and phytohormones on in vitro propagation of Larix decidua has been investigated. Needles, isolated vegetative buds, shoot tips, zygotic embryos and cotyledons were used as initial explants. Axillary and adventitious bud cultures were used for fast in vitro shoot multiplication. Root induction was stimulated on low salt medium supplemented with auxin. After rooting and hardening off, micropropagated trees were planted in experimental plots. The growth of micropropagated trees was comparable with the height and diameter growth of trees originated from seeds.

Keywords: Larix decidua; in vitro propagation; larch micropropagation; shoot multiplication; root induction; field growth of micropropagated trees

Published: December 31, 2004  Show citation

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Chalupa V. In vitro propagation of European larch (Larix decidua Mill.). J. For. Sci. 2004;50(12):553-558. doi: 10.17221/4656-JFS.
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    References

    1. BONGA J.M., 1984. Adventitious shoot formation in cultures of immature female strobili of Larix decidua. Physiol. Plant., 62: 416-421. Go to original source...
    2. BONGA J.M.,VON ADERKAS P., 1988. Attempts to micropropagate mature Larix decidua Mill. In: AHUJA M.R. (ed.), Somatic Cell Genetics of Woody Plants. Dordrecht, Kluwer: 155-168. Go to original source...
    3. CHALUPA V., 1983. Micropropagation of conifer and broadleaved forest trees. Commun. Inst. For. Czechosl., 13: 7-39.
    4. CHALUPA V., 1985. In vitro propagation of Larix, Picea, Pinus, Quercus, Fagus and other species using adenine-type cytokinins and thidiazuron. Commun. Inst. For. Czechosl., 14: 65-90.
    5. CHALUPA V., 1989. Micropropagation of Larix decidua Mill. and Pinus sylvestris L. Biol. Plant., 31: 400-407. Go to original source...
    6. CHALUPAV., 1990. Biotechnology in forest tree improvement: trees of the future. In: RODRIGUEZ R. et al. (eds.), Plant aging: basic and applied approaches. New York, Plenum Press: 311-318. Go to original source...
    7. CHALUPA V., 1991. Larch (Larix decidua Mill.) In: BAJAJ Y.P.S. (ed.), Biotechnology in Agriculture and Forestry, Vol. 16, Trees III. Berlin, Springer-Verlag: 446-470. Go to original source...
    8. CHALUPA V., ALDEN T., 1988. Control of morphogenesis by cytokinins in tissue cultures of forest tree species. In: HÄLLGREN J.E. (ed.), Molecular genetics of forest trees. Swed. Univ. Agric. Sci., Umea, Dep. Forest Genet., Report 8: 201-208.
    9. GRESSHOFF P.M., DOY C.H., 1972. Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta, 107: 161-170 Go to original source... Go to PubMed...
    10. HAKKILA P., WINTER A., 1974. On the properties of larch wood in Finland. Commun. Inst. For. Fenn., 79 (7): 1-45.
    11. KEITH C.T., CHAURET G., 1988. Basic wood properties of European larch from fast-growing plantations in eastern Canada. Can. J. For. Res., 18: 1325-1331. Go to original source...
    12. KLIMASZEWSKA K., 1989a. Plantlet development from immature zygotic embryos of hybrid larch through somatic embryogenesis. Plant Sci., 63: 95-103. Go to original source...
    13. KLIMASZEWSKA K., 1989b. Recovery of somatic embryos and plantlets from protoplast cultures of Larix eurolepis. Plant Cell Rep., 8: 440-444. Go to original source... Go to PubMed...
    14. LALIBERTÉ S., LALONDE M., 1988. Sustained organogenesis in callus cultures of Larix eurolepis initiated from short shoot buds of a 12-year-old tree. Am. J. Bot., 75: 767-777. Go to original source...
    15. LLOYD G., MC COWN B., 1980. Commercially-feasible micropropagation of moutain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb. Proc. Int. Plant Propagators Soc., 30: 421-427.
    16. MURASHIGE T., SKOOG F., 1962.Arevised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-497. Go to original source...
    17. NAGMANI R., BONGA J.M., 1985. Embryogenesis in subcultured callus of Larix decidua. Can. J. For. Res., 15: 1088-1091. Go to original source...
    18. QUOIRIN M., LEPOIVRE P., 1977. Etude de milieux adaptés aux cultures in vitro de Prunus. Acta Hort., 78: 437-442. Go to original source...

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