J. For. Sci., 2003, 49(9):403-411 | DOI: 10.17221/4713-JFS

Induced wound response of Norway spruce Picea abies P. Karst. after artificial inoculation by imagoes of Ips typographus

L. Jankovský, D. Novotný, R. Mrkva
1 Mendel University of Agriculture and Forestry, Faculty of Forestry and Wood Technology, Brno, Czech Republic
2 Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

Inoculation experiments were carried out on a set of trees with imagoes of Ips typographus L. which origin from the Šumava Mts. and the Křtiny Training Enterprise. The objective of back inoculations was to determine whether species found on the surface of Ips typographus imagoes spread after the inoculation also through host tissues. It the vicinity of inoculation by Ips typographus imagoes, marked necrotic zones are evident including symptoms of the penetration of vascular pathogens through phloem and sapwood. The most marked reactions were observed in case of inoculation by an untreated Ips typographus imago. Treatment of Ips typographus imagoes by Ibefungin and Fundazol preparations did not demonstrate expected effects in full scale. The spores of several ophiostomoid fungi like Ceratocystis polonica (Siem.) C. Moreau were observed on the surface of bark beetles and at the same time were re-isolated from wounds inoculated by Ips typographus imagoes. The other fungi like Ophiostoma bicolor Davidson & Wells, Leptographium cf. lundbergii Lagerberg & Melin., Pezicula eucrita Karst., Phomopsis sp. and other were found in wounds with the imagoes artificial infection.

Keywords: ophiostomoid fungi; pathogens; vascular tissues; Ceratocystis polonica; Ophiostoma bicolor; wound reaction; vascular mycosis; Ips typographus

Published: September 30, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Jankovský L, Novotný D, Mrkva R. Induced wound response of Norway spruce Picea abies P. Karst. after artificial inoculation by imagoes of Ips typographus . J. For. Sci. 2003;49(9):403-411. doi: 10.17221/4713-JFS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. BEAVER R.A., 1989. Insects - fungus relationships in the bark and ambrosia beetles. In: WILDING N., COLLINS N.M, HAMMOND P.M., WEBBER J.F (eds.), Insect - Fungus Interactions. San Diego, Academic Press: 119-143. Go to original source...
    2. CHRISTIANSEN E., 1985a. Ips ceratocystis infection of Norway spruce: What is a deadly dosage? Z. Angew. Ent., 99: 6-11. Go to original source...
    3. CHRISTIANSEN E., 1985b. Ceratocystis polonica inoculated in Norway spruce: Blue staining in relation to inoculum density, resinosis and tree growth. Eur. J. Path., 15: 160-167. Go to original source...
    4. CHRISTIANSEN E., 1992. After effects of drought did not predispose young Picea abies to infection by the bark beetletransmitted blue-stain fungus Ophiostoma polonicum. Scand. J. For. Res., 7: 557-569. Go to original source...
    5. CHRISTIANSEN E., SOLHEIM H., 1990. The bark beetleassociated blue-stain fungus Ophiostoma polonicum can kill various spruces and Douglas fir. Eur. J. For. Pathol., 20: 436-446. Go to original source...
    6. CHRISTIANSEN E., SOLHEIM H., 1994. Pathogenicity of five species of Ophiostoma fungi to Douglas fir. Medd. Skogforsk, Aas, 47: 1-12.
    7. FRANCKE-GROSMANN H., 1956. Hautdrusen als Trager der Pilzsymbiose bei Ambrosiakafern. Z. Morphol. Ökol., 45: 275-308. Go to original source...
    8. FRANCKE-GROSMANN H., 1967. Ectosymbiosis in Wood - Inhabiting Insects. Symbiosis 2. New York, London, Academic Press: 141-205. Go to original source...
    9. FISHER R.C., THOMPSON G.H., WEBB W.E., 1953. Ambrosia beetles in forests and sawmill. Part 1. Their biology, economic importance and control. Forestry Abstracts, 14: 381-389.
    10. FURNISS M.M., SOLHEIM H., CHRISTIANSEN E., 1990. Transmission of blue-stain fungi by Ips typographus (Coleoptera: Scolytidae) in Norway spruce. Ann. Entomol. Soc. Am., 83: 712-716. Go to original source...
    11. HORNTVEDT R., SOLHEIM H., 1991. Pathogenicity of Ophiostoma polonicum to Norway spruce: The effect of isolate age and inoculum dose. Paper of Norwegian Forest Research Institute No. 44 (4): 11.
    12. HORNTVEDT R., CHRISTIANSEN E., SOLHEIM H., WANG S., 1983. Artificial inoculation with Ips typographus - associated blue-stain fungi can kill healthy Norway spruce trees. Medd. Nor. Inst. Skogforsk., 38 (4): 1-20.
    13. JANKOVSKÝ L., MRKVA R., 1997. Přenos patogenů vaskulárních pletiv na smrku lýkožroutem smrkovým Ips typographus L. In: KRIŽOVÁ E., KODRÍK M. (eds.), Les, drevo, životné prostredie. Zbor. ref., Zvolen, TU: 209-218.
    14. JANKOVSKÝ L., MRKVA R., AMBROŽOVÁ L., 1998. Ranové reakce na infekci imágy Ips typographus. In: PAVLÍK M. (eds.), Mykoflóra pod vplyvom zmien životného prostredia. Zbor. ref., Zvolen, TU: 48-57.
    15. JANKOVSKÝ L., NOVOTNÝ D., MRKVA R., 2000. Doprovodná mykoflóra Ips typographus a ranové reakce smrku na umělou inokulaci imagy lýkožrouta smrkového. In: HLAVÁČ P., REINPRECH L., GÁPER J., Ochrana lesa a lesnícka fytopatológia. Zvolen, TU: 229-243.
    16. KIRISITS T., 1998. Pathogenicity of three blue-stain fungi associated with the bark beetle Ips typographus to Norway spruce in Austria - Wien. Österr. Z. Pilzkde, 7: 191-201.
    17. KIRISITS T., ANGLBERGER H., 1999. Report on a strain of the pathogenic blue-stain fungus Ceratocystis polonica with low virulence. Österr. Z. Pilzkde, 8: 157-167.
    18. KIRISITS T., OFFENTHALER I., 2000. Xylem sap flow on Norway spruce (Picea abies [L.] Karst.) after inoculation with the bark beetle transmitted blue-stain fungus Ceratocystis polonica. In: HASENAUER H. (ed.), Proc. from International Conference on Forest Ecosystem Restoration Ecological and Economical Impacts of Restoration Processes in Secondary Coniferous Forests 10.-12. April, 2000. Vienna: 151-153.
    19. KOTÝNKOVÁ-SYCHROVÁ E., 1966. Mykoflóra chodeb kůrovců v Československu. Čes. Mykol., 20: 45-53.
    20. KROKENE P., SOLHEIM H., 1996. Fungal associates of five bark beetle species colonizing Norway spruce. Can. J. For. Res., 26: 2115-2122. Go to original source...
    21. KROKENE P., SOLHEIM H., 1997. Growth of four bark beetle vectored blue-stain fungi in relation to the induced wound response in Norway spruce. Can. J. Bot., 75: 618-625. Go to original source...
    22. KROKENE P., SOLHEIM H., 1998. Phytopathogenicity of four blue-stain fungi associated with aggressive and non-aggressive bark beetles. Phytopathology, 88: 39-44. Go to original source... Go to PubMed...
    23. KROKENE P., 1996. The role of blue-stain fungi in tree-killing by bark beetles. [Ph.D. Thesis.] Oslo, Biologisk Inst. Universit: 115.
    24. LORIO P.L., HODGES J.D., 1985. Theories of interactions among bark beetles, associated microorganisms and host trees. Proc. Reprint of US Forest Service, New Orleans: 485-491.
    25. MRKVA R., JANKOVSKÝ L., 1996. Patogeni vaskulárních pletiv a jejich význam v procesu chřadnutí jehličnatých dřevin. Lesn. Práce, 75: 207-209.
    26. SHIGO A.L., 1991. Modern arboriculture: a system approach to the care of trees and their associates. Shigo and Trees, Associates Durham, New Hampshire: 423.
    27. SOLHEIM H., 1986. Species of Ophiostomataceae isolated from Picea abies infested by the bark beetle Ips typographus. Nord. J. Bot., 6: 199-207. Go to original source...
    28. SOLHEIM H., 1988. Pathogenicity of some Ips typographus associated blue stain fungi to Norway spruce. Aas, Paper of Norwegian Forest Research Institute, No 40 (14): 1-11.
    29. SOLHEIM H., 1991. Ecological aspects of fungi associated with the spruce bark beetle Ips typographus, with special emphasis on fungal invasion of Norway spruce sapwood and the role of the primary invader Ophiostoma polonicum. [Dr. Agric. Thesis.] Norwegian Forest Research Institute: 1-15.
    30. SOLHEIM H., 1993. Fungi associated with the spruce bark beetle Ips typographus in an edemic area in Norway. Scand. For. Res., 8: 118-122. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content