J. For. Sci., 2026, 72(1):14-27 | DOI: 10.17221/54/2025-JFS

Effect of livestock and vegetation structure on Carabidae (Coleoptera) diversity in dehesas of the Iberian PeninsulaOriginal Paper

Ana M. Cárdenas ORCID..., Carmen Torres ORCID..., Juan M. Hidalgo
Department of Zoology, Campus Rabanales, University of Córdoba, Córdoba, Spain

The response of carabid beetles to extensive livestock farming and vegetation structure in two traditionally managed 'dehesa' ecosystems was investigated. From March 2011 to January 2012, sampling was done, using pitfall trapping, on two forestry farms located in the Sierra de Hornachuelos Natural Park (Córdoba, Spain), both with hunting use, but one of them also with a heavy livestock load. On each forestry farm, two sampling plots were selected according to the vegetation structure. Faunal differences were proved through the ecological indices, and the faunal uniqueness was checked by the Coldwell and Coddington Complementarity Analysis. To identify the most influential factor on the carabid biodiversity, a Generalised Linear Mixed Model (GLMM) was performed. Results show that abundance and richness are higher in the plots with open vegetation, similarly like the number of recorded tribes. Nevertheless, the ecological indices do not reflect any significant differences. The complementarity between different vegetation structures exceeds that of the exploitation types. In fact, the GLMM analysis indicated that the livestock itself does not have a significant effect on the fauna. In addition, all exclusive, rare or endemic species came from the closed vegetation plots, suggesting that these areas may act as a reservoir of unique species in terms of biodiversity.

Keywords: biodiversity; carabids; forestry farm; ground beetles; oak forest

Received: July 18, 2025; Revised: December 16, 2025; Accepted: December 22, 2025; Prepublished online: January 29, 2026; Published: January 30, 2026  Show citation

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Cárdenas AM, Torres C, Hidalgo JM. Effect of livestock and vegetation structure on Carabidae (Coleoptera) diversity in dehesas of the Iberian Peninsula. J. For. Sci. 2026;72(1):14-27. doi: 10.17221/54/2025-JFS.
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References

  1. Atienza J.C., Zaballos J.M., Farinós G. (1995): Contribución al conocimiento de los carábidos corticícolas de la Península Ibérica (Coleoptera: Lebiidae). Boletín de la Asociación española de Entomología, 19: 197-199. (in Spanish)
  2. Baptista M., Carvalho R., Boieiro M., Bartz M., Mendes S., Timóteo S., Azevedo-Pereira H.M.V.S., Alves J., Alves da Silva A., Briones M.J.I., Serrano A.R.M., Sousa J.P., da Silva P.M. (2024): The role of sheltered habitats in biodiversity conservation of species sensitive to drought: A case study using ground beetles (Coleoptera, Carabidae) in the Gorongosa National Park. Biodiversity and Conservation, 33: 2345-236. Go to original source...
  3. Blondel J., Aronson J., Bodiou J.Y., Boeuf G. (2010): The Mediterranean Region: Biological Diversity in Space and Time. 2nd Ed. New York, Oxford University Press: 376.
  4. Bobbitt Z. (2021): Shannon Diversity Index: Definition and Example. Statology. Available at: https://www.statology.org/shannon-diversity-index/
  5. Bodí M.B., Cerdà A., Mataix-Solera J., Doer S.H. (2012): Efectos de los incendios forestales en la vegetación y el suelo en la cuenca mediterránea: Revisión bibliográfica. Boletín de la Asociación de Geógrafos Españoles, 58: 2058. (in Spanish) Go to original source...
  6. Borchard F., Buchholz S., Helbing F., Fartmann T. (2014): Carabid beetles and spiders as bioindicators for the evaluation of montane heathland restoration on former spruce forests. Biological Conservation, 178: 185-192. Go to original source...
  7. Boticario E.F.J. (2011): La Revolución Neolítica y su influencia en los paisajes forestales en las áreas montañosas de Cantabria. In: Boticario E.F.J., van den Bercken E.R. (eds): La evolución del paisaje vegetal y el uso del fuego en la Cordillera Cantábrica. León, Fundación Patrimonio Natural de Castilla y León: 91-108. Available at: https://dialnet.unirioja.es/servlet/articulo?codigo=7457703 (in Spanish)
  8. Cárcamo H.A., Spence J. (1994): Crop type effects on the activity and distribution of ground beetles (Coleoptera: Carabidae). Environmental Entomology, 23: 684-692. Go to original source...
  9. Carrión J.S., Munuera M., Ochando J., López-Sáez J.A., Casas-Gallego M., González-Sampériz P., Badal E., Pérez-Díaz S., Carrión-Marco Y., Jiménez-Moreno G. et al. (2022): Paleoflora y Paleovegetación Ibérica III: Holoceno. Murcia, Ministerio de Ciencia e Innovación y Fundación Séneca. Available at: https://www.paleofloraiberica.org/wp-content/uploads/2023/03/VOLUMEN-3-HOLOCENO-I-2.pdf (in Spanish)
  10. Casale A. (2000): Natural history and applied ecology of carabid beetles. In: Proceedings of the IX European Carabidiologists Meeting, Cosenza, Jul 26-31, 1998: 26-31.
  11. Colwell R.K., Coddington J.A. (1994): Estimating terrestrial biodiversity through extrapolation. Philosophical Transactions of the Royal Society of London. Series B, 345: 101-118. Go to original source... Go to PubMed...
  12. Eyre M.D., Luff M.L. (1990): A preliminary classification of European grassland habitats using carabid beetles. In: Stork N.E. (ed.): The Role of Ground Beetles in Ecological and Environmental Studies. Andover, Intercept: 227-236.
  13. Frenette-Dussault C., Shipley B., Hingrat Y. (2013): Linking plant and insect traits to understand multitrophic community structure in arid steppes. Functional Ecology, 27: 786-792. Go to original source...
  14. Giller P.S., Hillebrand H., Berninger U.G., Gessner M.O., Hawkins S., Inchausti P., Inglis C., Leslie H., Malmqvist B., Monaghan M.T., Morin P.J., O'Mullan G. (2004): Biodiversity effects on ecosystem functioning: Emerging issues and their experimental test in aquatic environments. Oikos, 104: 423-436. Go to original source...
  15. Halme E., Niemelä J. (1993): Carabid beetles in fragments of coniferous forest. Annales Zoologici Fennici, 30: 17-30.
  16. Hammer Ø., Harper D.A.T., Ryan P.D. (2001): PAST: Paleontological statistics software package for education and data analysis. Paleontologica Electronica, 4: 1-9.
  17. Hidalgo J.M., Cárdenas A.M. (2024): Descripción de un nuevo Platyderus Stephens, 1827 (Coleoptera, Carabidae) del medio subterráneo de Córdoba (sur de la península ibérica). Boletín de la Asociación Española de Entomología, 48: 219-228. (in Spanish) Go to original source...
  18. Holland J.M. (2002): The Agroecology of Carabid Beetles. Andover, Intercept Limited: 356.
  19. Honbein R.R., Connawy C.J. (2018): Pitfall traps: A review of methods for estimating arthropod abundance. Wildlife Society Bulletin, 42: 597-606. Available at: https://www.jstor.org/stable/26558988 Go to original source...
  20. Honek A. (1997): The effect of temperature on the activity of Carabidae (Coleoptera) in a fallow field. European Journal of Entomology, 94: 97-104.
  21. Karacetin E., Durmus M., Yilmaz K., Lise Y. (2022): Effects of grazing and mowing on butterfly diversity, a case study from the east of Turkey. In: Asian Grassland Conference, online, Apr 19-21, 2022: 27. Available at: https://www.iavs.org/events/EventDetails.aspx?id=1574658&group=
  22. Knapp M., ©trobl M., Venturo A., Seidl M., Jakubíková L., Tajovský K., Kadlec T., González E. (2022): Importance of grassy and forest non-crop habitat islands for overwintering of ground-dwelling arthropods in agricultural landscapes: A multi-taxa approach. Biological Conservation, 275: 109757. Go to original source...
  23. Kosewska A., Kêdzior R., Nietupski M., Borkowski J. (2023): Epigeic Carabids (Coleoptera, Carabidae) as bioindicators in different variants of Scots pine regeneration: Implication for forest landscape management. Sustainability, 15: 13322. Go to original source...
  24. Kotze D.J., Brandmayr P., Casale A., Dauffy-Richard E., Dekoninck W., Koivula M.J., Lövei G.L., Mossakowski D., Noordijk J., Paarmann W., Pizzolotto R., Saska P., Schwerk A., Serrano J., Szyszko J., Taboada A., Turin H., Venn S., Vermeulen R., Zetto T. (2011): Forty years of carabid beetle research in Europe from taxonomy, biology, ecology and population studies to bioindication, habitat assessment and conservation. Zookeys, 100: 55-148. Go to original source... Go to PubMed...
  25. Laguna E. (2025): Estimación de la rareza y grado de amenaza de las especies. Available at: https://www.uv.es/elalum/CursIVAPLSerraText.pdf (accessed June 13, 2025; in Spanish)
  26. Lindroth C.H. (1961): The ground-beetles (Carabidae, excl. Cicindelinae) of Canada and Alaska. Part 2. Opuscula Entomologica, Suppl. 20: 1-200.
  27. Lindroth C.H. (1963): The ground-beetles (Carabidae, excl. Cicindelinae) of Canada and Alaska. Part 3. Opuscula Entomologica, Suppl. 24: 201-408.
  28. Lindroth C.H. (1966): The ground-beetles (Carabidae, excl. Cicindelinae) of Canada and Alaska. Part 4. Opuscula Entomologica, Suppl. 29: 409-648.
  29. Lindroth C.H. (1968). The ground-beetles (Carabidae, excl. Cicindelinae) of Canada and Alaska. Part 5. Opuscula Entomologica, Suppl. 33: 649-944.
  30. Lindroth C.H. (1969): The ground-beetles (Carabidae, excl. Cicindelinae) of Canada and Alaska. Part 6. Opuscula Entomologica, Suppl. 34: 945-1192.
  31. Lövei G.L., Sunderland K.D. (1996): Ecology and behaviour of ground beetles (Coleoptera: Carabidae). Annual Review of Entomology, 41: 231-256. Go to original source... Go to PubMed...
  32. Lozano-Parra J., Pulido M., Lozano-Fondón C., Schnabel S. (2018): How do soil moisture and vegetation covers influence soil temperature in drylands of Mediterranean regions? Water, 10: 1747. Go to original source...
  33. Martín-Piera F. (2000): Estimaciones prácticas de biodiversidad utilizando táxones de alto rango en insectos. In: Martín-Piera F., Morrone J.J., Melic A. (eds): Hacia un proyecto CYTED para el Inventario y Estimación de la Diversidad Entomológica en Iberoamérica: PriBES 2000. m3m-Monografías Tercer Milenio, 1. Zaragoza, Sociedad Entomológica Aragonesa: 36-54. (in Spanish)
  34. Moreno G., Aviron S., Berg J., Crous-Duran A., de Jalón C.J., Hartel J., Mirck A., Pantera J.H.N., Palma J.H.N., Paulo J.A., Re J.A., Sanna F., Thenail C., Varga A., Viaud V., Burgess P.J. (2018): Agroforestry systems of high nature and cultural value in Europe: Provision of commercial goods and other ecosystem services. Agroforestry Systems, 92: 877-891. Available at: https://link.springer.com/article/10.1007/s10457-017-0126-1 Go to original source...
  35. Mullen K., O'Halloran J., Breen J., Giller P., Pithon J., Kelly T. (2008): Distribution and composition of carabid beetle (Coleoptera, Carabidae) communities across the plantation forest cycle - Implications for management. Forest Ecology and Management, 256: 624-632. Go to original source...
  36. Pardo R. (2023): Aplicación del análisis pedoantracológico para la reconstrucción del área de distribución del paleoendemismo Abies pinsapo. [Ph.D. Thesis] Granada, University of Granada. Available at: https://hdl.handle.net/10481/85724 (in Spanish)
  37. Peña L., Zapata L. (2012): Las transformaciones económicas del Neolítico en la Península Ibérica. In: Rojo M.A., Garrido R., García-Martínez de Lagrán I. (eds): El Neolítico en la Península Ibérica y su contexto europeo. Madrid, Catédra: 95-106. Available at: https://dialnet.unirioja.es/servlet/articulo?codigo=4145954 (in Spanish)
  38. Picaud F., Petit D.P. (2007): Primary succession of Orthoptera on mine tailings: Role of vegetation. Annales de la Société Entomologique de France, 43: 69-79. Go to original source...
  39. Pizzolotto R. (2022): Carabids species diversity in Mediterranean beech forests. Biodiversity Data Journal, 10: e78291. Go to original source... Go to PubMed...
  40. Plieninger T., Wilbrand C. (2001): Land use, biodiversity conservation, and rural development in the dehesas of Cuatro Lugares, Spain. Agroforestry Systems, 51: 23-34. Available at: https://ltsermontado.pt/?q=Publications&f%5Bkeyword%5D=2404 Go to original source...
  41. Pozsgai G., Littlewood N.K. (2014): Ground beetle (Coleoptera: Carabidae) population declines and phenological changes: Is there a connection? Ecological Indicators, 41: 15-24. Go to original source...
  42. R Core Team R. (2020): A language and environment for statistical computing. Vienna, R Foundation for Statistical Computing. Available at: https://www.r-project.org/
  43. Rainio J., Niemelä J. (2003): Ground beetles (Coleoptera: Carabidae) as bioindicators. Biodiversity and Conservation, 12: 487-506. Go to original source...
  44. Ramakrishna G.B., Murthy J.S.V., Sushma G., Thanu C.N. (2024): Perspective Chapter: Ground Dwelling Carabids - Challenges and Conservation in a Dynamic Environment. In: Hågvar S., Ødegaard F. (eds): Insect Conservation: Challenges and Possibilities in a Changing World. London, Intech Open: 79-98. Go to original source...
  45. Rischen T., Frenzel T., Fischer K. (2021): Biodiversity in agricultural landscapes: Different non-crop habitats increase diversity of ground-dwelling beetles (Coleoptera) but support different communities. Biodiversity and Conservation, 30: 3965-3981. Go to original source...
  46. Robinson S.I., McLaughlin Ó.B., Marteinsdóttir B., O'Gorman E.J. (2018): Soil temperature effects on the structure and diversity of plant and invertebrate communities in a natural warming experiment. Journal of Animal Ecology, 87: 634-646. Go to original source... Go to PubMed...
  47. Rodríguez-Pajares C., Muñoz-Adalia E.J., Fernández-Fernández M. (2025): Microarthropods communities as indicators of soil quality in a Mediterranean periurban forest using the QBS-ar index. Forest Systems, 34: 20906. Go to original source...
  48. Rodríguez-Rojo M.P., Roig S., López-Carrasco C., Redondo-García M.M., Sánchez-Mata D. (2022): Which factors favour biodiversity in Iberian dehesas? Sustainability, 14: 2345. Go to original source...
  49. Rumohr Q., Baden C.U., Bergtold M., Marx M.T., Oellers J., Schade M., Toschki A., Maus C. (2023): Drivers and pressures behind insect decline in Central and Western Europe based on long-term monitoring data. PLoS One, 18: e0289565. Go to original source... Go to PubMed...
  50. Sánchez-Martín J.M., Blas-Morato R., Rengifo-Gallego J.I. (2019): The dehesas of Extremadura, Spain: A potential for socio-economic development based on agritourism activities. Forests, 10: 620. Go to original source...
  51. Saska P., van der Werf W., Hemerik L., Luff M.L., Hatten T.D., Honek A. (2013): Temperature effects on pitfall catches of epigeal arthropods: A model and method for bias correction. Journal of Applied Ecology, 50: 181-189. Go to original source... Go to PubMed...
  52. Serrano-Montes J.L., Martínez-Ibarra E., Arias-García J. (2019): How does the presence of livestock influence landscape preferences? An image-based approach. Landscape Online, 71: 1-18. Go to original source...
  53. SGPC (2022): La conservación de la dehesa y las intervenciones de desarrollo rural en el PEPAC 2023-2027. Ministry of Agriculture, Fisheries and Food, Sub-Directorate General for Programming and Coordination. Available at: https://www.mapa.gob.es/es/pac/pac-2023-2027/la-conservacion-de-la-dehesa-y-las-intervenciones-de-desarrollo-rura_tcm30-626885.pdf (accessed June 13, 2025; in Spanish)
  54. Taboada A., Kotze D.J., Salgado J.M., Tárrega R. (2006): The influence of habitat type on the distribution of carabid beetles in traditionally managed 'dehesa' ecosystems in NW Spain. Entomologica Fennica, 17: 284-295. Go to original source...
  55. Talarico F., Giglio A., Pizzolotto R., Brandmayr P. (2016): A synthesis of feeding habits and reproduction rhythm in Italian see-fee ground beetles (Coleoptera: Carabidae). European Journal of Entomology, 113: 325-336. Go to original source...
  56. Thiele H.U. (1977): Carabid beetles in their environments: A study on habitat selection by adaptations in physiology and behaviour. Berlin, Springer-Verlag: 369.
  57. Thomas S.R. (2002): How to protect or what we know about Carabid beetles: From knowledge to application, from Wijster (1969) to Tuczno (2001). In: Szyszko J., den. Boer P.J., Bauer T. (eds): The Refuge Role of Beetle-Banks and Field Margins for Carabid Beetles on UK Arable Farmland: Densities, Composition and Relationships with Vegetation. Warsaw, Warsaw Agricultural University Press: 185-199.
  58. Torralba M., Fagerholm N., Hartel T., Moreno G., Plieninger T. (2018): A social-ecological analysis of ecosystem services supply and trade-offs in European wood-pastures. Science Advances, 4: eaar2176. Go to original source...
  59. Tsafack N., Xie Y., Wang X., Fattorini S. (2019): Influence of climate and local habitat characteristics on carabid beetle abundance and diversity in northern Chinese steppes. Insects, 11: 19. Go to original source... Go to PubMed...
  60. Van der Plas F., Anderson T.M., Olff H. (2012): Trait similarity patterns within grass and grasshopper communities: Multitrophic community assembly at work. Ecology, 93: 836-846. Go to original source... Go to PubMed...
  61. Verdú J.R., Moreno C.E., Sánchez-Rojas G., Numa C., Galante E., Halffter G. (2007): Grazing promotes dung beetle diversity in the xeric landscape of a Mexican biosphere reserve. Biological Conservation, 140: 308-317. Go to original source...
  62. Walker B.H. (1992): Biodiversity and ecological redundancy. Conservation Biology, 6: 18-23. Go to original source...
  63. Weiss F., Winter S., Pflugmacher D., Kolling T., Linde A. (2024): Evidence for regional-scale declines in carabid beetles in old lowland beech forests following a period of severe drought. Landscape Ecology, 39: 123. Go to original source...

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