J. For. Sci., 2016, 62(6):269-278 | DOI: 10.17221/15/2016-JFS
Increasing drought resistance of Alnus subcordata C.A. Mey. seeds using a nano priming technique with multi-walled carbon nanotubesOriginal Paper
- 1 Department of Arid Land Forestry, Faculty of Desert Studies, Semnan University, Semnan, Iran
- 2 Young Researchers and Elite Club, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran
- 3 Department of Engineering of Wood and Paper Industries, Faculty of Natural Resources, Semnan University, Semnan, Iran
To evaluate the effects of nano priming on seed germination of Alnus subcordata (Caucasian alder) C.A. von Meyer under drought stress, the present research was conducted using multi-walled carbon nanotubes (MWCNTs; 0, 10, 30, 50, and 100 mg.l-1) at 6 levels of drought stress (0, -2, -4, -6, -8, and -10 bar) through a factorial experiment (5 priming levels with 4 replications). After priming, the seeds were placed into a germinator at 21°C. Results revealed that nano priming at the concentration of 100 mg.l-1 led to the highest germination rate and percentage at all levels of drought stress. Also, the highest values of seed vigour index and root and stem lengths and dry weights were observed at nano carbon treatment with 30 mg.l-1. Considering the obtained results, it was concluded that nano priming could result in boosted resistance of Caucasian alder seeds against drought stress, so that the seed tolerance increased from -4 bar (without nano priming treatment, i.e. reference sample) to -8 bar upon applying 100 mg.l-1 nanotubes. Based on the results of the present research, it is suggested that the seed nano priming technique with MWCNTs can be applied in order to increase the seed and seedling tolerance of other members of the genus Alnus Miller.
Keywords: polyethylene glycol 6000; seed vigour index; seed coat permeability; drought tolerance; alder tree; nano carbon
Published: June 30, 2016 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Adhikari T., Kundu S., Rao A.S. (2013): Impact of SiO2 and Mo nano particles on seed germination of rice (Oryza sativa L.). International Journal of Agriculture Food Science & Technology, 4: 809-816.
- Afzal I., Rauf S., Basra S.M.A., Murtaza G. (2008): Halopriming improves vigor, metabolism of reserves and ionic contents in wheat seedlings under salt stress. Plant, Soil and Environment, 54: 382-388.
Go to original source... - Ashraf M., Foolad M.R. (2005): Pre-sowing seed treatment - a shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy, 88: 223-271.
Go to original source... - Baalbaki R.Z., Zurayk R.A., Blelk M.M., Tahouk S.N. (1999): Germination and seedling development of drought tolerant and susceptible wheat under moisture stress. Seed Science and Technology, 27: 291-302.
- Batool A., Ziaf K., Amjad M. (2015): Effect of halo-priming on germination and vigor index of cabbage (Brassica oleracea var. capitata). Journal of Environmental and Agricultural Sciences, 2: 1-8.
- Benson D.R., Silvester W.B. (1993): Biology of Frankia strains, actinomycete symbionts of actinorhizal plants. Microbiological Reviews, 57: 293-319.
Go to original source...
Go to PubMed... - Bradbeer J.W. (1988): Seed Dormancy and Germination. Glasgow, Blackie and Son Ltd.: 146.
Go to original source... - Bradford K.J. (1995): Water relations in seed germination. Seed Development and Germination, 1: 351-396.
Go to original source... - Brancalion P.H.S., Novembre A.D.L.C., Rodrigues R.R., Tay D. (2008): Priming of Mimosa bimucronata seeds: A tropical tree species from Brazil. Acta Horticulturae, 782: 163-168.
Go to original source... - Cañas J.E., Long M., Nations S., Vadan R., Dai L., Luo M., Ambikapathi R., Lee E.H., Olszyk D. (2008): Effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of select crop species. Environmental Toxicology and Chemistry, 27: 1922-1931.
Go to original source...
Go to PubMed... - Chai M.W., Shi F.C., Li R.L., Liu L.M., Liu Y., Liu F.C. (2013): Interactive effects of cadmium and carbon nanotubes on the growth and metal accumulation in a halophyte Spartina alterniflora (Poaceae). Plant Growth Regulation, 71: 171-179.
Go to original source... - Chen Z., Li J. (2004): Phylogenetics and biogeography of Alnus (Betulaceae) inferred from sequences of nuclear ribosomal DNA region. International Journal of Plant Sciences, 165: 325-335.
Go to original source... - Chen Z.D., Manchester S.R., Sun H.Y. (1999): Phylogeny and evolution of the Betulaceae as inferred from DNA sequences, morphology, and paleobotany. American Journal of Botany, 86: 1168-1181.
Go to original source... - Claessens H., Oosterbaan A., Savill P., Rondeux J. (2010): A review of the characteristics of black alder (Alnus glutinosa (L.) Gaertn.) and their implications for silvicultural practices. Forestry, 83: 163-175.
Go to original source... - Dehkourdi E.H., Mosavi M. (2013): Effect of anatase nanoparticles (TiO2) on parsley seed germination (Petroselinum crispum) in vitro. Biological Trace Element Research, 155: 283-286.
Go to original source...
Go to PubMed... - Haghighi M., da Silva J.T. (2014): The effect of carbon nanotubes on the seed germination and seedling growth of four vegetable species. Journal of Crop Science and Biotechnology, 17: 201-208.
Go to original source... - Haghighi M., Pessarakli M. (2013): Influence of silicon and nano-silicon on salinity tolerance of cherry tomatoes (Solanum lycopersicum L.) at early growth stage. Scientia Horticulturae, 161: 111-117.
Go to original source... - ISTA (2009): ISTA Handbook on Seedling Evaluation. 3rd Ed. Zurich, International Seed Testing Association: 117.
- Kaldenholff R., Fischer M. (2006): Aquaporins in plants. Acta Physiologica, 187: 169-176.
Go to original source...
Go to PubMed... - Katerji N., van Hoorn J.W., Hamdy A., Karam F., Mastrorilli M. (1994): Effect of salinity on emergence and on water stress and early seedling growth of sunflower and maize. Agricultural Water Management, 26: 81-91.
Go to original source... - Kaya M.D., Okçu G., Atak M., Çikili Y., Kolsarici Ö. (2006): Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24: 291-295.
Go to original source... - Khodakovskaya M., Dervishi E., Mahmood M., Xu Y., Li Z., Watanabe F., Biris A.S. (2009): Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS Nano, 3: 3221-3227.
Go to original source...
Go to PubMed... - Khodakovskaya M.V., de Silva K., Biris A.S., Dervishi E., Villagarcia H. (2012): Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano, 6: 2128-2135.
Go to original source...
Go to PubMed... - Khot L.R., Sankaran S., Maja J.M., Ehsani R., Schuster E.W. (2012): Applications of nanomaterials in agricultural production and crop protection: A review. Crop Protection, 35: 64-70.
Go to original source... - Kulkarni M.G., Street R.A., Staden J.V. (2007): Germination and seedling growth requirements for propagation of Diosscorea dregeana (Kunth) Dur. and Schinz - a tuberous medicinal plant. South African Journal of Botany, 73: 131-137.
Go to original source... - Kumar R., Shamet G.S., Alam N.M., Jana C. (2016): Influence of growing medium and seed size on germination and seedling growth of Pinus gerardiana Wall. Compost Science & Utilization, 24: 98-104.
Go to original source... - Larue C., Pinault M., Czarny B., Georgin D., Jaillard D., Bendiab N., Carrière M. (2012): Quantitative evaluation of multi-walled carbon nanotube uptake in wheat and rapeseed. Journal of Hazardous Materials, 227: 155-163.
Go to original source...
Go to PubMed... - Lin D., Xing B. (2007): Phytotoxicity of nanoparticles: Inhibition of seed germination and root growth. Environmental Pollution, 150: 243-250.
Go to original source...
Go to PubMed... - Liu H.Y., Yu X., Cui D.Y., Sun M.H., Sun W.N., Tang Z.C., Su W.A. (2007): The role of water channel proteins and nitric oxide signaling in rice seed germination. Cell Research, 17: 638-649.
Go to original source...
Go to PubMed... - Liu Q., Chen B., Wang Q., Shi X., Xiao Z., Lin J., Fang X. (2009): Carbon nanotubes as molecular transporters for walled plant cells. Nano Letters, 9: 1007-1010.
Go to original source...
Go to PubMed... - Maurel C. (2007): Plant aquaporins: Novel functions and regulation properties. FEBS Letters, 581: 2227-2236.
Go to original source...
Go to PubMed... - Merouropoulos G., Bernett D.C., Shitsat A.H. (1999): The Arabidopsis extensin gene is developmentally regulated, is induced by wounding, methyl jasmonate, abscisic and salicylic acid, and codes for a protein with unusual motifs. Planta, 208: 212-219.
Go to original source...
Go to PubMed... - Michel B.E., Kaufmann M.R. (1973): The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51: 914-916.
Go to original source...
Go to PubMed... - Monica R.C., Cremonini R. (2009): Nanoparticles and higher plants. Caryologia, 62: 161-165.
Go to original source... - Nair R., Varghese S.H., Nair B.G., Maekawa T., Yoshida Y., Kumar D.S. (2010): Nanoparticulate material delivery to plants. Plant Science, 179: 154-163.
Go to original source... - Samaj J., Baluska F., Voigt B., Schlicht M., Volkmann D., Menzel D. (2004): Endocytosis, actin cytoskeleton, and signaling. Plant Physiology, 135: 1150-1161.
Go to original source...
Go to PubMed... - Sharma P., Sardana V., Banga S.S. (2013): Salt tolerance of Indian mustard (Brassica juncea) at germination and early seedling growth. Environmental and Experimental Botany, 11: 39-46.
- Siddiqui M.H., Al-Whaibi M.H. (2014): Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum seeds Mill.). Saudi Journal of Biological Sciences, 21: 13-17.
Go to original source...
Go to PubMed... - Song J., Fan H., Zhao Y., Jia Y., Du X., Wang B. (2008): Effect of salinity on germination, seedling emergence, seedling growth and ion accumulation of a euhalophyte Suaeda salsa in an intertidal zone and on saline inland. Aquatic Botany, 88: 331-337.
Go to original source... - Taniguchi T., Kataoka R., Futai K. (2008): Plant growth and nutrition in pine (Pinus thunbergii) seedlings and dehydrogenase and phosphatase activity of ectomycorrhizal root tips inoculated with seven individual ectomycorrhizal fungal species at high and low nitrogen conditions. Soil Biology & Biochemistry, 40: 1235-1243.
Go to original source... - Tiré C., De Rycke R., De Loose M., Inzé D., Van Montagu M., Engler G. (1994): Extensin gene expression is induced by mechanical stimuli leading to local cell wall strengthening in Nicotiana plumbaginifolia. Planta, 195: 175-181.
Go to original source...
Go to PubMed... - Torney F., Trewyn B.G., Lin V.S.Y., Wang K. (2007): Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nature Nanotechnology, 2: 295-300.
Go to original source...
Go to PubMed... - Tripathi S., Sonkar S.K., Sarkar S. (2011): Growth stimulation of gram (Cicer arietinum) plant by water soluble carbon nanotubes. Nanoscale, 3: 1176-1181.
Go to original source...
Go to PubMed... - Wang X., Han H., Liu X., Gu X., Chen K., Lu D. (2012): Multiwalled carbon nanotubes can enhance root elongation of wheat (Triticum aestivum) plants. Journal of Nanoparticle Research, 14: 1-10.
Go to original source...
Go to PubMed... - Yuan H.G., Hu S.L., Huang P., Song H., Wang K., Ruan J., He R., Cui D.X. (2011): Single walled carbon nanotubes exhibit dual-phase regulation to exposed Arabidopsis mesophyll cells. Nanoscale Research Letters, 6: 44-52.
Go to original source...
Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.

